| 甘南州尖顶羊肚菌菌丝体液体发酵培养研究 |
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| 引用本文： | 康小虎,蔡亚东,孙宜法,谢放,任爱梅,孟宪刚.甘南州尖顶羊肚菌菌丝体液体发酵培养研究[J].中国食用菌,2012(6):40-43. |
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| 作者姓名： | 康小虎 蔡亚东 孙宜法 谢放 任爱梅 孟宪刚 |
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| 作者单位： | 兰州交通大学化学与生物工程学院生物工程系,甘肃兰州730070 |
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| 摘 要： | 对甘南藏族自治州尖顶羊肚菌进行液体发酵培养，以干菌丝体得率为指标，设计正交试验优化其液体发酵培养基和培养条件。实验结果表明最佳培养基组合为白砂糖30g·L^-1、豆粕粉15g·L^-1、KH2PO4 0．75g·L^-1、MgSO4·7H2O0．5g·L^-1、维生素Bt0．03g·L^-1。最优液体发酵条件为温度25℃，培养液初始pH7，接种量10mL，转速120r·min^-1，培养时间8d。
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| 关 键 词： | 甘南藏族自治州 尖顶羊肚菌 液体发酵 |
Study on Liquid Fermentation of Mycelia of Morchella conica in Gannan Autonomous Prefecture |
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| KANG Xiao-hu,CAI Ya-dong,SUN Yi-fa,XIE Fang,REN Ai-mei,MENG Xian-gang.Study on Liquid Fermentation of Mycelia of Morchella conica in Gannan Autonomous Prefecture[J].Edible Fungi of China,2012(6):40-43. |
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| Authors: | KANG Xiao-hu CAI Ya-dong SUN Yi-fa XIE Fang REN Ai-mei MENG Xian-gang |
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| Institution: | (School of Chemistry and Bioengineering, Lanzhou Jiaotong University, Lanzhou Gansu 730070) |
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| Abstract: | The medium and liquid fermentation conditions of Morchella conica which growed in Gannan Tibetan au- tonomous prefecture was studied in the paper as the index to dry mycelia yield by orthogonal experiment. The results showed that the best medium components was sugar （30g·L^-1）, soybean powder （15 g·L^-1）, KH2PO4 （0.75 g·L^-1）, Mg- SO4·7H2O （0.5 g·L^-1） and vitamin Bl （0.03 g·L^-1）. The optimal conditions of liquid fermentation were showed as follows： the temperature was 25℃, the initial pH was 7, the strains for inoculation quantity was 10 mL, the rotate speed was 120 r· min^-1 and culture time was 8 days. |
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| Keywords: | Gannan tibetan autonomous prefecture Morchella conica Liquid fermentation |
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