| 水稻Rubisco活化酶的提取和纯化 |
| |
| 引用本文： | 翁晓燕,蒋德安,陆庆,毛伟华.水稻Rubisco活化酶的提取和纯化[J].浙江大学学报(农业与生命科学版),1999(3). |
| |
| 作者姓名： | 翁晓燕 蒋德安 陆庆 毛伟华 |
| |
| 作者单位： | 浙江大学生物系!浙江杭州310029 |
| |
| 基金项目： | 国家自然科学基金!39570432 |
| |
| 摘 要： | 利用３５％饱和硫酸铵分部沉淀、ＤＥＡＥ－ｃｅｌｌｕｌｏｓｅ和ＭｏｎｏＱ柱层析等步骤从水稻叶片纯化了 Ｒｕｂｉｓｃｏ活化酶。 ＳＤＳ－ＰＡＧＥ电泳显示水稻Ｒｕｂｉｓｃｏ活化酶由４５ｋＤ和４１ ｋＤ两个亚基组成。 ＡＴＰ是该 酶活化Ｒｕｂｉｓｃｏ的必需成份。
|
| 关 键 词： | 水稻 Rubisco活化酶 提取 纯化 |
Purification of Rubisco activase from rice. |
| |
| WENG Xiao-yan,JIANG De-an,LU Qing,MAO Wei-hua.Purification of Rubisco activase from rice.[J].Journal of Zhejiang University(Agriculture & Life Sciences),1999(3). |
| |
| Authors: | WENG Xiao-yan JIANG De-an LU Qing MAO Wei-hua |
| |
| Abstract: | Rubisco 1. 5-bisphosphate carboxylase/oxygenase activase was purified from rice leaves by 35% ammonium sulfate fraction, DEAE-cellulose and Mono Q chromatography. SDS-PAGE electrophore- togram showed that Rubisco activase (RCA) was composed of 45 kD and 41 kD subunits. ATP was neces- sary to maintain Rubisco activase activity. Maximal activity of the enzyme was obtained with ATP 1. 5 mmol/L. |
| |
| Keywords: | rice Rubisco activase extraction purification |
| 本文献已被 CNKI 等数据库收录！ |
