| 家兔精液冷冻保存的研究 |
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| 引用本文： | 娄安钢,李继鹏,崔成都.家兔精液冷冻保存的研究[J].延边大学农学学报,2009,31(1):57-61. |
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| 作者姓名： | 娄安钢 李继鹏 崔成都 |
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| 作者单位： | 延边大学农学院动物医学系,吉林,龙井,133400 |
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| 摘 要： | 以家兔为试验动物，通过精液在4℃保存时间来筛选合适的基础液，并从精液解冻后的精子活率和活力2个指标筛选出最佳的平衡时间和抗冻剂（甘油）浓度．冷冻保护液中渗透性抗冻剂（甘油）的3种浓度为3％，4％和5％．4℃平衡时间分别为2，3，4h．将3种甘油浓度的冷冻保护液与3种平衡时间相配伍，共9组进行冷冻试验，筛选出稀释液为：Tris3．03g，柠檬酸1．30g，果糖1．50g，卵黄20%，青、链霉素各1×10^5U，双蒸水100mL．冷冻后结果表明，3％的甘油浓度平衡3h，解冻后精子活力在0．3以上，达到人工授精的要求．
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| 关 键 词： | 家兔 精液 冷冻保存 |
The Study on Rabbit Semen Cryopreservation |
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| LOU An-gang,LI Ji-peng,CUI Cheng-du.The Study on Rabbit Semen Cryopreservation[J].Journal of Agricultural Science Yanbian University,2009,31(1):57-61. |
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| Authors: | LOU An-gang LI Ji-peng CUI Cheng-du |
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| Institution: | (Agricultural College of Yanbian University ,Longjing Jilin 133400,China) |
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| Abstract: | Take the Rabbit as experimental animal. Screening the appropriate foundation fluid through the seminal fluid in 4℃ retention time, and according to frozen-thawing sperm recovery and mobility rate of sperm screens the best equilibrium time and the antifreeze （glycerine） density. In freezing protection fluid permeable antifreeze （glycerine） three kinds of densities was 3%, 4% and 5%, 4℃ equilibrium times was 2,3 and 4 h, respectively. Combining three kinds of glycerine densities freezing protection fluid with three kinds of equilibrium time, 9 groups were studied. Screened the diluent is tris 3.03 g, citric acid 1.30 g,fructose 1.50 g,yelk 20%, penicillin 1×10^5U, streptomycin 1×10^5 U,D. W 100 mL. The freezing results showed that 3% glycerine density balance for 3h, frozen-thawing sperm mobility above 0.3. Achieved the artificial insemination the request. |
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| Keywords: | Rabbit semen cryopreservation |
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