| 十溴联苯醚对秀丽隐杆线虫毒性研究 |
| |
| 引用本文： | 王赢利,陈建松,阳宇翔,解启来.十溴联苯醚对秀丽隐杆线虫毒性研究[J].土壤学报,2015,52(5):1116-1123. |
| |
| 作者姓名： | 王赢利 陈建松 阳宇翔 解启来 |
| |
| 作者单位： | 广东嘉应学院,广东嘉应学院,华南农业大学资源环境学院 |
| |
| 基金项目： | 广东省自然科学基金项目（S2013010013809） |
| |
| 摘 要： | 十溴联苯醚(BDE-209)是一类新型持久性有机污染物,可导致神经系统和生殖发育等多种生理毒性。本文以秀丽隐杆线虫(Caenorhabditis elegans)为模式生物,通过分析十溴联苯醚(BDE-209)对其繁殖、寿命、细胞凋亡以及体内超氧化物歧化酶基因sod-3、类p-53蛋白基因cep-1、细胞色素P450基因cyp35a2以及谷胱甘肽转移酶基因gst-1的影响,来评价BDE-209的生理、细胞及分子水平毒性。试验结果表明,与空白对照相比,BDE-209低剂量(5 mg kg-1)短时间暴露,对秀丽线虫细胞凋亡及产卵影响不显著;高浓度长时间暴露(30 mg kg-1)会导致秀丽隐杆线虫产卵数目下降,寿命缩短,细胞凋亡。Real-time PCR试验表明,低剂量(5 mg kg-1)暴露使得sod-3、cep-1、cyp35a2基因表达显著增加;高剂量暴露(30 mg kg-1),虽然sod-3表达显著增加,但增加幅度低于低浓度暴露组,cep-1、cyp35a2基因表达显著受到抑制。说明长期暴露在高浓度(30 mg kg-1)十溴联苯醚环境中,会促使秀丽隐杆线虫产生氧化应激反应,秀丽隐杆线虫通过调控相关抗氧化基因的表达来修复这种损伤,而高浓度的BDE-209会造成其机体氧化损伤、细胞凋亡及产卵量下降。
|
| 关 键 词： | 十溴联苯醚 秀丽隐杆线虫 繁殖 抗氧化酶 基因表达 |
| 收稿时间： | 2015/1/14 0:00:00 |
| 修稿时间： | 2015/4/14 0:00:00 |
Toxicity of deca-brominated diphenyl ether to Caenorhabditis elegans |
| |
| Wang Yingli,Chen Jiansong,Yang Yuxiang and Xie Qilai.Toxicity of deca-brominated diphenyl ether to Caenorhabditis elegans [J].Acta Pedologica Sinica,2015,52(5):1116-1123. |
| |
| Authors: | Wang Yingli Chen Jiansong Yang Yuxiang and Xie Qilai |
| |
| Institution: | School of Chemistry and Environment,Jiaying University,School of Chemistry and Environment,Jiaying University,School of Chemistry and Environment,Jiaying University |
| |
| Abstract: | Abstract: Deca-brominated diphenyl ether (BDE-209) is a new kind of persistent organic pollutant, which may have toxicity to nervous system and reproductionon. In the present study, Caenorhabditis elegans was chosen as a model organism. Effects of BDE-209 on its reproduction, life time, and cells of BDE-209, and its gene expression of sod-3 (superoxide dismutase-3), cep-1 (tumor proteins p53-1), cyp35a2 (cytochrome P450 35a2) and GST-1 (glutathione-S-transferase-1) were investigated to evaluate toxicity of the substance to its physiology and cells at the molecular level. Results show that compared with CK, short exposure to low dosage (5 mg kg-1) of BDE-209 did not have much effect on cell apoptosis and oviposition, but exposed to a higher dosage (30 mg kg-1) for a longer time, Caenorhabditis elegans would have lower ovipositing capacity, shorter life time and cell apoptosis. Real-time PCR demonstrates that exposure to a low dosage ( 5 mg kg-1) of BDE-209 significantly enhanced the gene expression of sod-3, cep-1, and cyp35a2, however, exposure to a high dosage would also enhance the expression of sod-3, though not so apparent as in the former, but significantly reduce the expressions of cep-1 and cyp35a2. These findings indicate that, when exposed to a high dosage of BDE-209 for a long time, C. Caenorhabditis elegans would generate oxidative stress trying to repair the damage through regulating the expression of related anti-oxidation genes, but BDE-209 high in concentration would still cause oxidative injury and cell apoptosis to its body, and reduce its oviposition capacity. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《土壤学报》浏览原始摘要信息 |
| 点击此处可从《土壤学报》下载免费的PDF全文 |
|
