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类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响
引用本文:温立斌,何孔旺,杨汉春,郭容利,钟书霖,周俊明,陈梦海.类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(Z1).
作者姓名:温立斌  何孔旺  杨汉春  郭容利  钟书霖  周俊明  陈梦海
作者单位:1. 江苏省农业科学院,兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏南京,210014
2. 中国农业大学动物医学院,北京,100094
3. 扬州大学生物科学与技术学院,江苏扬州,225009
基金项目:国家"973"计划前期研究专项,江苏省自然科学基金 
摘    要:探讨类猪圆环病毒因子P1感染对猪细胞免疫功能的影响.将12头30日龄广西巴马小型猪随机分成试验组和对照组,每组6头.在猪感染P1后第3,8,17,24,31和40天,采用血常规和单色流式细胞术分析猪外周血T淋巴细胞的含量,通过WST法对其增殖活性进行检测.P1感染组的T淋巴细胞数量(绝对数和相对数)整体趋势低于对照组,而两组的T淋巴细胞增殖活性没有显著差别.P1感染对机体的细胞免疫功能有一定程度的影响.

关 键 词:类猪圆环病毒因子  广西巴马小型猪  T淋巴细胞计数  T淋巴细胞增殖活性

Effects of Porcine Circovirus-like Agent P1 Infection on Counts and Proliferation Activity of T Lymphocyte in Peripheral Blood of Piglets
WEN Li-bin,HE Kong-wang,YANG Han-chun,GUO Rong-li,ZHONG Shu-lin,ZHOU Jun-ming,CHEN Meng-hai.Effects of Porcine Circovirus-like Agent P1 Infection on Counts and Proliferation Activity of T Lymphocyte in Peripheral Blood of Piglets[J].Acta Agriculturae Boreali-Sinica,2009,24(Z1).
Authors:WEN Li-bin  HE Kong-wang  YANG Han-chun  GUO Rong-li  ZHONG Shu-lin  ZHOU Jun-ming  CHEN Meng-hai
Institution:WEN Li-bin1,HE Kong-wang1,YANG Han-chun2,GUO Rong-li1,ZHONG Shu-lin1,ZHOU Jun-ming1,CHEN Meng-hai3(1.Institute of Veterinary Medicine,Jiangsu Academy of Agriculture Sciences,Key Laboratory of Animal Diseases Diagnostic and Immunology,Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China,2.College of Veterinary Medicine,China Agricultural University,Beijing 100094,3.College of Biological Science and Technology,Yangzhou University,Yangzhou 22500...
Abstract:The research is to explore the effects of porcine circovirus-like agent P1 infection on counts and proliferation activity of T lymphocyte in peripheral blood of piglets.Twelve 30-day-old guangxi bama miniature pigs were randomly divided into two groups of six animals each.A complete haemogram and single-color flow cytometry was performed to determine the T lymphocyte of 3,8,17,24,31 and 40 days after inoculation.meanwhile,WST assay was to do the T lymphocyte proliferation activity.There was no significant d...
Keywords:Porcine circovirus-like agent  Guangxi bama miniature pig  T lymphocyte count  T lymphocyte proliferation activity  
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