| 人血清白蛋白基因的克隆及序列分析 |
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| 引用本文： | 李虎,贺小英,杨瑞峰,李向臣,华松,张涌.人血清白蛋白基因的克隆及序列分析[J].安徽农业科学,2005,33(5):801-802. |
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| 作者姓名： | 李虎 贺小英 杨瑞峰 李向臣 华松 张涌 |
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| 作者单位： | 西北农林科技大学生物工程研究所,陕西,杨凌,712100;西北农林科技大学生物工程研究所,陕西,杨凌,712100;西北农林科技大学生物工程研究所,陕西,杨凌,712100;西北农林科技大学生物工程研究所,陕西,杨凌,712100;西北农林科技大学生物工程研究所,陕西,杨凌,712100;西北农林科技大学生物工程研究所,陕西,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 根据Genebank(登陆号V00495)中人血清白蛋白HSA的cDNA序列,设计扩增引物。采用RTPCR自人胎盘中扩增出HSA基因的全长cDNA。产物纯化后连至PMD18T载体,转化大肠杆菌DH5α。筛选阳性克隆菌,酶切鉴定并进行序列测定。结果表明,该试验成功地克隆了HSA基因的1995bp全长cDNA,与Genebank中参照序列同源性为99%。
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| 关 键 词： | 人血清白蛋白(HSA) RT-PCR 序列测定 胎盘 |
| 文章编号： | 0517-6611(2005)05-0801-02 |
Clone and Sequence of Human Serum Albumin cDNA from Human Placenta |
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| LI Hu et al.Clone and Sequence of Human Serum Albumin cDNA from Human Placenta[J].Journal of Anhui Agricultural Sciences,2005,33(5):801-802. |
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| Authors: | LI Hu |
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| Abstract: | According to the cDNA sequence of HSA(human serum albumin HSA) derived from the Genebank(V00495), a pair of primers was designed. RT-PCR was applied to amplify the whole cDNA of HSA gene. The DNA fragment was cloned into vector pMD-18T and transformed into E.coli DH5α host bacteria. XbaⅠendonuclease digestion and sequenced from selected positive cloned bacteria. The results showed that the whole 1995 bp cDNA of HSA were cloned successfully and compared with the sequence deposited in Gene Banks, and the homology was 99%. |
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| Keywords: | Human serum albumin (HSA) RT-PCR Sequence Placenta |
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