| 猪苓多糖苯酚-硫酸法测定条件的优选 |
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| 引用本文： | 田广文,陈德育,李学俊,舒志明.猪苓多糖苯酚-硫酸法测定条件的优选[J].中国农学通报,2007,23(7):75-75. |
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| 作者姓名： | 田广文 陈德育 李学俊 舒志明 |
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| 作者单位： | 西北农林科技大学生命学院,陕西杨凌,712100 |
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| 摘 要： | 【目的】建立猪苓多糖快速定量分析方法；【方法】苯酚-硫酸法，以葡萄糖含量为指标，检测波长490nm，用L9（34）正交试验设计优选测定条件；【结果】1.0ml样液，加5%苯酚液0.8ml、硫酸5.0ml，100℃水浴保温15min结果最佳；硫酸加量对结果影响最大，水浴温度次之，苯酚量影响最小；葡萄糖含量呈线性关系范围在5.0～40.0μg/ml；精密度RSD=1.58%(n=6)；测定稳定性好(3h内)；平均回收率99.22％，RSD=1.87％（n=3）；实测猪苓菌丝多糖含量3.46%，RSD=2.07%(n=4)。【结论】本优选法灵敏度高、操作简便、数据稳定性好、误差小、重现性好，值得推广
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| 关 键 词： | 烟草 烟草 钾营养 研究进展 |
| 修稿时间： | 2007-04-052007-04-18 |
Optimization of Phenol-Sulfuric Acid Determination Conditions of Polysaccharide
in Polyporus Umbellatus by Orthogonal Test |
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| Tian Guangwen,Cheng Deyu,Li xuejun,Shu zhiming.Optimization of Phenol-Sulfuric Acid Determination Conditions of Polysaccharide
in Polyporus Umbellatus by Orthogonal Test[J].Chinese Agricultural Science Bulletin,2007,23(7):75-75. |
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| Authors: | Tian Guangwen Cheng Deyu Li xuejun Shu zhiming |
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| Institution: | (College of Life Science, Northwest A & F University, Yangling Shannxi 712100, China) |
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| Abstract: | 【Objective】To establish rapid quantitative analysis method for polysaccharide in Polyporus umbellatus.【Method】Taking phenol - sulfuric acid method to content of glucose as parameters. The absorbance is measured at 490 nm. L9 (34) Orthogonal Design optimum determine conditions. 【Results】when the sample solution used 1.0ml, 0.8 ml of 5% solution of phenol, and sulfuric acid 5.0ml, 100 ° C water bath for 15 min reaction conditions determination, the results is best. Also sulfuric acid increment to result influence biggest, the water bath temperature is next, the phenol quantity influence is smallest. The glucose had good linear relationship in 5.0～40.0μg/ml. RSD=1.58% precision (n = 6), measured 3h good stability. The average of recovery was 99.22%, RSD=1.87% (n = 3). The content of polysaccharide in Polyporus umbellatus was measured 3.46%, RSD=2.07% (n = 4). 【Conclusion】This optimization method is highly sensitive, simple, stable data, accurate, good reproducibility and worth promoting. |
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| Keywords: | Polysaccharide in Polyporus umbellatus Phenol-sulfuric acid method Determination condition orthogonal optimal |
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