| 不同季节水牛卵母细胞体外受精效果的研究 |
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| 引用本文： | 黄芬香,庞春英,张秀芳,覃广胜,陈明棠,杨春艳,李日聪,李嫜,梁贤威.不同季节水牛卵母细胞体外受精效果的研究[J].中国畜牧兽医,2008,35(7):76-78. |
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| 作者姓名： | 黄芬香 庞春英 张秀芳 覃广胜 陈明棠 杨春艳 李日聪 李嫜 梁贤威 |
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| 作者单位： | 中国农业科学院水牛研究所，南宁 530001 |
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| 基金项目： | 农业部948项目
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广西创新能力建设项目
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桂科转0718005-3C |
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| 摘 要： | 利用屠宰场收集水牛卵巢回收卵母细胞，比较了不同季节的水牛卵母细胞体外受精的效果。试验连续进行了2年，按水牛发情的规律，1年的试验数据分3个阶段统计。结果表明，水牛发情旺季（8~11月）平均每个水牛卵巢回收可用卵母细胞3.66枚，明显高于发情淡季的4~7月和12月~次年3月两个季节（分别为2.76和3.07枚，P＜0.01）；而在不同季节回收的水牛卵母细胞体外受精后分裂率和囊胚率差异不显著（P＞0.05）。
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| 关 键 词： | 季节 水牛 卵母细胞 体外受精 |
In Vitro Fertilization and Subsequent Embryo Development Using Oocytes Derived from Abattoir Ovaries in Different Seasons in Buffalo |
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| HUANG Fen-xiang,PANG Chun-ying,ZHANG Xiu-fang,QIN Guang-sheng,CHEN Ming-tang,YANG Chun-yan,LI Ri-cong,LI Zhuo,LIANG Xian-wei.In Vitro Fertilization and Subsequent Embryo Development Using Oocytes Derived from Abattoir Ovaries in Different Seasons in Buffalo[J].China Animal Husbandry & Veterinary Medicine,2008,35(7):76-78. |
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| Authors: | HUANG Fen-xiang PANG Chun-ying ZHANG Xiu-fang QIN Guang-sheng CHEN Ming-tang YANG Chun-yan LI Ri-cong LI Zhuo LIANG Xian-wei |
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| Institution: | Institute of Buffalo Research，Chinese Academy of Agriculture Sciences，Nanning 530001，China |
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| Abstract: | The buffalo oocytes were derived from abattoir ovaries,the effects of IVF of oocytes in different seasons were compared.The experiment has been lasted for two years,the data were divided into tree groups and statistically analyzed according to the oestrum cycle in buffalo.The results indicated that an average of 3.66 usable oocytes were derived from an ovary in oestrum season(Aug-Nov) which was significant higher than those in Mar-Jul and Dec-Feb(2.76 and 3.07 respectively,P<0.01).The cleavage rate and blastocyst rate of IVF oocytes obtained in different season showed no difference(P>0.05). |
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| Keywords: | season buffalo oocyte IVF |
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