| 中国近海真鲷遗传变异的线粒体控制区序列分析 |
| |
| 引用本文： | 乐小亮,章群,赵爽,范凤娟.中国近海真鲷遗传变异的线粒体控制区序列分析[J].广东农业科学,2010,37(2):136-139. |
| |
| 作者姓名： | 乐小亮 章群 赵爽 范凤娟 |
| |
| 作者单位： | 暨南大学水生生物研究所,广东,广州,510632 |
| |
| 基金项目： | 国家自然科学基金(30770415);;教育部留学归国人员科研启动基金(2005) |
| |
| 摘 要： | 测定了辽宁东港、广东大亚湾和广西东兴3个海域各15尾真鲷的线粒体控制区5′端660bp的核苷酸序列,发现91个可变位点,64个简约信息位点。在45尾样品中共检测到43个单倍型,3个群体的平均单倍型多样性和核苷酸多样性分别为(0.99～1)和(0.02522～0.02579),表现出较高的单倍型多样性和核苷酸多样性。在真鲷个体NJ树上出现3个谱系,各谱系中不同地理来源的个体比例相当,不呈现明显的地理聚群,推测各谱系大约形成于晚更新世的末期。Tajima’sD呈负值但不显著(P0.08),表明真鲷历史上没有发生快速扩张,种群大小稳定。群体间遗传分化指数Fst都为负值(-0.0001～-0.0188)但不显著(P0.1);AMOVA分析显示遗传变异主要集中在群体内的个体间(100.49%),而群体间的遗传变异很小(-0.49%);表明我国近海真鲷群体有可能是同一种群。
|
| 关 键 词： | 真鲷 遗传多样性 线粒体控制区 |
Genetic variation of red seabream (Pagrus major) in coastal waters of China inferred from mitochondrial DNA control region sequence analysis |
| |
| YUE Xiao-liang,ZHANG Qun,ZHAO Shuang,FAN Feng-juan.Genetic variation of red seabream (Pagrus major) in coastal waters of China inferred from mitochondrial DNA control region sequence analysis[J].Guangdong Agricultural Sciences,2010,37(2):136-139. |
| |
| Authors: | YUE Xiao-liang ZHANG Qun ZHAO Shuang FAN Feng-juan |
| |
| Institution: | Research Center of Hydrobiology;Jinan University;Guangzhou 510632;China |
| |
| Abstract: | To analyze genetic variation of red seabream population in coastal waters of China, 660 bp DNA sequences at 5'-terminal mtDNA control region of 45 individuals sampled from 3 localities including Doaggang in Liaoling province, Dayawang in Guangdong province, and Dongxin in Guangxi province were determined. Totally 91 variable sites and 64 parsimony informative sites defined 43 haplotypes among 45 individuals. The average of haplotype diversity and nucleotide diversity among 3 geographic group were 0.99～1 and 0.0252～0.0257 respectively, indicating both high haplotype and nucleotide diversity. In the Neighbor-Joining tree 3 lineages were found without obvious geographic groups, as the ratio of individuals from different locality within each lineages were similar. The deduced speciation time of 3 lineages were at the end of late Pleistocene. Tajima's D were negative but not significant(P>0.08), indicating a stable population size without rapid expanding. Pair-wise genetic differentiation index Fst were negative (-0.00148～-0.01770) but not significant (P>0.1);genetic variation within and among populations were 100.49% and -0.49% respectively in AMOVA analysis;it were suggested that red seabream populations in Donggang, Dayawan and Dongxiag along coastal waters of China might be treated as one population. |
| |
| Keywords: | Pagrus major genetic diversity mtDNA control region |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
