Structure and antioxidant activity study of sulfated acetamido-polysaccharide from Radix Hedysari |
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| Institution: | 1. School of Pharmacy, Lanzhou University, 199 Donggangxi Road, Lanzhou 730000, PR China;2. The Second People''s Hospital of Gansu Province, Hezhengxi Road 388, Lanzhou 730000, PR China;1. Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China;2. Analytical and Testing Center, Beihua University, Jilin 132013, China;3. School of Pharmacy, Beihua University, Jilin 132013, China;4. College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China;1. School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China;2. Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China;3. University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, PR China;4. School of Life Sciences, Shanghai University, No. 99, Shangda Road, Shanghai 200444, PR China |
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| Abstract: | A new sulfated acetamido-heteropolysaccharide, HPS4-2A, was obtained by aqueous extraction followed by precipitation with ethanol and fractionation with DEAE column chromatography from Radix Hedysari. It was composed of rhamnose, arabinose, glucose, galactose and 2-acetamido-2-deoxy-d-galactose in the molar ratio of 10.09%:25.90%:25.90%:25.0%:12.30%. Elemental analysis indicated that HPS4-2A was a sulfated polysaccharide containing small amount of sulfate groups (1.87%). Partial acid hydrolysis, GC, GC–MS, 1D and 2D NMR spectroscopy analysis of the HPS4-2A revealed a predominance of glucose, galactose and 2-acetamido-2-deoxy-d-galactose linked in a highly-branched structure. The molecular weight of HPS4-2A was determined by HPSEC and HPSEC-MALLS. AFM study indicated that HPS4-2A took a highly branched conformation, which in consistent with the result studied by SEC-MALLS. Structural features of HPS4-2A were also investigated by SEM and TEM. Antioxidant assays demonstrated that HPS4-2A possessed of strong DPPH and hydroxyl radicals scavenging activities, suggesting that HPS4-2A could potentially be used as natural antioxidant. |
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