| 结球甘蓝—大白菜异源三倍体小孢子培养的研究 |
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| 引用本文： | 张巍巍,申书兴,王彦华,陈雪平,轩淑欣,罗双霞,李晓峰.结球甘蓝—大白菜异源三倍体小孢子培养的研究[J].园艺学报,2009,36(4):583-586. |
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| 作者姓名： | 张巍巍 申书兴 王彦华 陈雪平 轩淑欣 罗双霞 李晓峰 |
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| 作者单位： | (河北农业大学园艺学院, 河北保定071001) |
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| 基金项目： | 国家自然科学基金，河北省自然科学基金 |
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| 摘 要： | 为创建结球甘蓝—大白菜异附加系, 以大白菜二倍体(AA, 2n=2x=20) 与结球甘蓝四倍体(CCCC, 2n=4x=36) 杂交获得的8个异源三倍体(ACC, 2n=3x=28) 单株系为试材, 进行游离小孢子培养研究, 获得再生植株, 并对其进行了染色体数目鉴定和性状调查。结果表明, 异源三倍体(ACC) 的小孢子诱导成胚的能力低于双亲; 在NLN中添加15%的蔗糖和0.1 mg·L-1 6-BA、0.2 mg·L-1 2, 4-D、0.05 mg·L-1 KT的培养基上小孢子胚胎发生最高为0.0250个·蕾-1, 8个株系间有差异, 平均为0.0100个·蕾-1; 胚胎再生率只有33.3%。小孢子再生植株染色体数在14～27条之间, 田间性状表现多样。
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| 关 键 词： | 结球甘蓝 大白菜 小孢子培养 胚状体 异附加系 |
| 收稿时间： | 2008-10-17 |
| 修稿时间： | 2009-3-24 |
Studies on Isolated Microspore Culture of the Allotriploid Hybrids Between Cabbage and Chinese Cabbage |
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| ZHANG Wei-wei,SHEN Shu-xing,WANG Yan-hua,CHEN Xue-ping,XUAN Shu-xin,LUO Shuang-xia,LI Xiao-feng.Studies on Isolated Microspore Culture of the Allotriploid Hybrids Between Cabbage and Chinese Cabbage[J].Acta Horticulturae Sinica,2009,36(4):583-586. |
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| Authors: | ZHANG Wei-wei SHEN Shu-xing WANG Yan-hua CHEN Xue-ping XUAN Shu-xin LUO Shuang-xia LI Xiao-feng |
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| Institution: | (College of Horticulture, Agricultural University of Hebei, B aoding, Hebei 071001, China) |
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| Abstract: | Microspore culture of 8 allotriploid hybrids between diploid Chinese cabbage (AA, 2n=2x=20) and autotetrap loid cabbage (CCCC, 2n=4x=36) was performed in order to create the alien addition lines. The results showed that embryo p roduction frequency of the allotrip loid hybrids (ACC) was lower thantheir parents. In liquid medium NLN with 15% sucrose and 0.1 mg·L-1 6-BA, 0.2 mg·L-1 2, 4-D, 0.05mg·L-1 KT, the 8 lines were different, the highest of embryo production was 0.0250 embryos per bud, the average was 0.0100 embryos per bud. The p lant regeneration frequency of embryoids was only 33.3%. The chromosome number of plantlets derived from microspore culture varied from 14 to 27. The morphological characters were diverse among the different plants. |
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| Keywords: | cabbage Chinese cabbage microspore culture embryo alien addition lines |
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