| 双阻NH4+选择性微电极测定水稻叶片细胞中NH4+的活度 |
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| 引用本文： | 尹晓明,范晓荣,沈其荣.双阻NH4+选择性微电极测定水稻叶片细胞中NH4+的活度[J].植物营养与肥料学报,2009,15(3):701-706. |
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| 作者姓名： | 尹晓明 范晓荣 沈其荣 |
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| 作者单位： | 1.南京农业大学资源与环境科学学院,江苏省固体有机废弃物资源化利用高技术研究重点实验室,江苏南京 210095 |
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| 摘 要： | 本文介绍了双阻NH4+选择性微电极的制作方法、工作原理及操作方法。微电极电位响应値与溶液中NH4+的活度呈对数曲线的关系，NH4+选择性微电极与其他类型的电极(如H+、NO3-)最大区别是K+的干扰，在含有72 mmol/L K+的标定溶液中，电极标定曲线的斜率为48~58 mV，对NH4+的检出限小于10-4 mol/L，说明电极对NH4+有较高的选择性，受K+的影响较小，可以用来测定。用以测定2.5 mmol/L NH4+培养2周的水稻叶片，结果表明，叶片细胞中NH4+活度分布在高低不同的两个区间内，分别代表了细胞质和液泡中的测定，水稻叶片细胞质和液泡NH4+的活度分别为2.58~9.30
mmol/ L和11.36~25.20 mmol/L。水稻叶片组织中的NH4+主要来自液泡，流动分析仪测定的水稻叶片组织的NH4+浓度为11.12 mmol/L。NH4+选择性微电极为研究水稻对NH4+的吸收利用提供了技术支撑。
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| 关 键 词： | 双阻NH4+ style="FONT-SIZE: 9pt FONT-FAMILY: 宋体 mso-font-kerning: 1.0pt mso-ansi-language: EN-US mso-fareast-language: ZH-CN mso-bidi-language: AR-SA mso-ascii-font-family: mso-hansi-font-family: ms |
| 收稿时间： | 2008-4-8 |
Measurement of NH4+;activity in leaf cells of rice using double-barreled NH4+selective microelectrodes |
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| YIN Xiao-ming,FAN Xiao-meng,SHEN Qi-rong.Measurement of NH4+;activity in leaf cells of rice using double-barreled NH4+selective microelectrodes[J].Plant Nutrition and Fertilizer Science,2009,15(3):701-706. |
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| Authors: | YIN Xiao-ming FAN Xiao-meng SHEN Qi-rong |
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| Institution: | 1.College of Recourses and Environmental Science,Jiangsu Provincial Key Lab of Organic Solid Waste Utilization,Nanjing Agricultural University,Nanjing 210095,China; |
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| Abstract: | This paper reported the manufacture method, working principle and operating method of double-barreled NH4+ selective microelectrode. An apparent difference of NH4+ selective microelectrode from other microelectrodes (such as H+ and NO3- microelectrode) is the interference of K+. These microelectrodes showed a typical log linear response to NH4+ from 0.01–100 mmol/L in the presence of 72mmol/L K+, with a slope of 48 ~58 mV. The detect limit was below 10-3mmol/L. The results showed that the microelectrode was more sensitive and selective to NH4+ and could be used on the NH4+ measurement. Intracellular measurement of NH4+ activity in leaf cells of rice using the double-barreled NH4+ selective microelectrode showed that NH4+ activity fell into two main populations, one was in the cytoplasm with the NH4+concentrations of 2.58–9.37 mmol/L and the other one was in the vacuole with the NH4+concentrations of 11.36–25.20 mmol/L. Tissue NH4+ of rice leaf mainly comes from that of the vacuole. Tissue NH4+ concentrations of rice leaf using a continuous-flow auto analyzer were 11.12mmol/L. NH4+ selective microelectrode offered technical support for the study of the assimilation and utilization of NH4+ in rice. |
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| Keywords: | double-barreled NH4+-selective microelectrodes rice leaf cell NH4+ |
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