| 禽流感病毒N3亚型神经氨酸酶基因的克隆 |
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| 引用本文： | 邓国华,王秀荣,张立春,刘振勇,乔传玲,田国彬,于康震,陈化兰.禽流感病毒N3亚型神经氨酸酶基因的克隆[J].中国预防兽医学报,2004,26(3):161-164. |
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| 作者姓名： | 邓国华 王秀荣 张立春 刘振勇 乔传玲 田国彬 于康震 陈化兰 |
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| 作者单位： | 中国农业科学院,哈尔滨兽医研究所,农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 本研究应用11日龄SPF鸡胚增殖禽流感病毒标准株A/Duck/Germany/1215(H2N3),用LSTRIAZOLL试剂盒提取病毒RNA,应用自行设计的NA基因半特异性引物进行RT-PCR扩增,得到NA基因的全长DNA片段,克隆到PMD18-T载体上,并进行鉴定和序列测定.测定结果表明所获得的NA基因DNA片段长1451bp,编码469个氨基酸残基,与其他亚型的NA基因编码的氨基酸长度相一致.根据推导的氨基酸序列进行预测,该蛋白具有6个潜在的糖基化位点和19个半胱氨酸残基.与其他亚型的NA基因相比较发现克隆的NA基因符合神经氨酸的分子结构特征,本研究首次获得N3亚型NA基因全序列.
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| 关 键 词： | 禽流感病毒 神经氨酸酶 N3NA基因 克隆 |
| 文章编号： | 1008-0589(2004)03-0161-04 |
| 修稿时间： | 2003年7月9日 |
Cloning and analysis of N3 subtype neuraminidase of avian influenza virus |
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| DENG Guo-hua WANG Xiu-rong ZHANG Li-chun LIU Zhen-yongQIAO Chuan-ling TIAN Guo-bin YU Kang-zhen CHEN Hua-lan.Cloning and analysis of N3 subtype neuraminidase of avian influenza virus[J].Chinese Journal of Preventive Veterinary Medicine,2004,26(3):161-164. |
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| Authors: | DENG Guo-hua WANG Xiu-rong ZHANG Li-chun LIU Zhen-yongQIAO Chuan-ling TIAN Guo-bin YU Kang-zhen CHEN Hua-lan |
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| Institution: | DENG Guo-hua WANG Xiu-rong ZHANG Li-chun LIU Zhen-yongQIAO Chuan-ling TIAN Guo-bin YU Kang-zhen CHEN Hua-lan~* |
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| Abstract: | A N3 subtype influenza virus A/Duck/Germany/1215/73(H2N3) was grown in 11-day-old SPF embryonated chicken eggs.Virial RNA was isolated by treatment of aliquots of the virus with LS TRIAZOL.Full-length NA cDNA was prepared by RT-PCR and cloned into pMDT-18 vector.Sequences proved that the cloned NA gene consist of 1495 nucleotides and encode a polypeptide of 469 amino acids residues.The N3 NA protein has 6 potential glycosylation site and 19 cysteines.The cloned gene has the same molecular character of influenza NA gene compare to the other subtypes neuraminidase. |
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| Keywords: | influenza A virus neuraminidase N3 subtype cloning |
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