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| 利用AFLP标记技术评价甘蓝型油菜的遗传多样性和亲缘关系 | |
| 引用本文： | 李丽,黄先群,WanapuChokchai,雷尊国,黄团,李其义,彭义,黄贵民,董颍苹.利用AFLP标记技术评价甘蓝型油菜的遗传多样性和亲缘关系[J].西南农业学报,2011,24(4). |
| 作者姓名： | 李丽 黄先群 WanapuChokchai 雷尊国 黄团 李其义 彭义 黄贵民 董颍苹 |
| 作者单位： | 1. 贵州省生物技术研究所,贵州省农业生物技术重点实验室,贵州贵阳550006;泰国苏兰拉里理工大学农学院生物技术系,呵叻泰国30000 2. 贵州省生物技术研究所,贵州省农业生物技术重点实验室,贵州贵阳550006 3. 泰国苏兰拉里理工大学农学院生物技术系,呵叻泰国30000 4. 贵州省种子管理站 贵州贵阳550002 |
| 基金项目： | 贵州省科技攻关项目“甘蓝型油菜含油量基因的QTL分析”[黔科合NY字(2007)3006]; 贵州省科学技术基金项目“贵州马铃薯栽培品种的SRAP分析”[黔科合J字(2010)2084]~~ |
| 摘 要： | 利用AFLP分子标记技术分析了25份甘蓝型油菜品种的遗传多样性.9对AFLP引物扩增出193条带,其中,73条呈多态性,多态性比率为38％.每对引物检测出的平均等位基因数为3.56,有效等位基因数为1.41.基因多样性、香农指数和遗传差异分别为0.25、0.62和0.39.在遗传相似系数0.66处,所有的甘蓝型油菜可分为3个类群,遗传相似系数表明,橄榄型油菜表现出丰富的遗传多样性.UPGMA聚类分析表明,品种间的亲缘关系与种质性状或来源关系不明显. |
| 关 键 词： | AFLP 甘蓝型油菜 遗传多样性 |
Assessment of Genetic Diversity and Relationships among Brassica napus Using AFLP Markers |
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| LI Li,HUANG Xian-qun,WanapuChokchai,LEI Zun-guo,HUANG Tuan,LI Qi-yi,PENG Yi,HUANG Gui-min,DONG Ying-ping.Assessment of Genetic Diversity and Relationships among Brassica napus Using AFLP Markers[J].Southwest China Journal of Agricultural Sciences,2011,24(4). | |
| Authors: | LI Li HUANG Xian-qun WanapuChokchai LEI Zun-guo HUANG Tuan LI Qi-yi PENG Yi HUANG Gui-min DONG Ying-ping |
| Institution: | LI Li1,2,HUANG Xian-qun1,Wanapu Chokchai2,LEI Zun-guo1,HUANG Tuan1,LI Qi-yi3,PENG Yi3,HUANG Gui-min3,DONG Ying-ping1 (1.Guizhou Institute of Biotechnology,Guizhou Key Laboratory of Agricultural Biotechnology,Guizhou Guiyang 550006,China,2.School of Biotechnology,Suranaree University of Technology,Nakon Ratchasima 30000,Thailand,3.Guizhou Seed Management Station,Guizhou Guiyang 550002,China) |
| Abstract: | AFLP markers were applied to assess genetic diversity and relationships among 25 B.napus cultivars.Nine AFLP primer combinations generated 193 fragments,of which 73 were polymorphic.The polymorphic rate was 38％.The average number of observed alleles per locus was 3.56,while the mean number of effective alleles was 1.41.Gene diversity,Shannon' s information index,and genetic differentiation were 0.25,0.62,and 0.39,respectively.At a genetic similarity coefficient of 0.66,all B.napus cultivars could be divided into three groups.A wide range of similarity coeffcient value suggested that the investigated B.napus germplasm collection represented a genetically diverse population.UPGMA cluster analysis revealed that no apparent clustering by traits or original locations. |
| Keywords: | AFLP B napus Genetic diversity |
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