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| 小麦种子水分胁迫萌发的基因差异表达 | |
| 引用本文： | 郭继虎,景蕊莲,王金胜.小麦种子水分胁迫萌发的基因差异表达[J].山西农业大学学报(自然科学版),2003,23(1):23-27. |
| 作者姓名： | 郭继虎 景蕊莲 王金胜 |
| 作者单位： | 1. 中国农业科学院作物品种资源研究所,农业部作物种质资源与生物技术重点实验室,北京,100081；山西农业大学文理学院,山西,太谷,030801 2. 中国农业科学院作物品种资源研究所,农业部作物种质资源与生物技术重点实验室,北京,100081 3. 山西农业大学文理学院,山西,太谷,030801 |
| 摘 要： | 旱选10号为材料,以PEG-6000为胁迫荆,利用DDRT-PCR技术研究了种子芽期水分胁迫处理和对照材料在mRNA表达上的差异。获得的5个差异表达片段,在处理前后表达丰度均呈增加趋势,并为反向Northern点杂交所验证。经查询,2个片段与GenBank中已知基因有较高同源性,3个片段与已知的EST序列同源。 |
| 关 键 词： | 小麦 水分胁迫 差异显示 |
| 文章编号： | 1671-8151(2003)01-0023-05 |
| 修稿时间： | 2002年9月13日 |
Differential Display of mRNA Induced by Water Stress in Germinating Wheat (Triticum aestivum L. ) |
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| GUO Ji-hu et al..Differential Display of mRNA Induced by Water Stress in Germinating Wheat (Triticum aestivum L. )[J].Journal of Shanxi Agricultural University,2003,23(1):23-27. | |
| Authors: | GUO Ji-hu |
| Abstract: | In this paper, wheat variety HanxuanlO was used as a plant material and PEG-6000 solution as water stress agent. The differential expression of mRNA of the samples between the treatments and the control during the seed germination was analyzed with DDRT-PCR method. 5 differential expressed cDNA Fragments were obtained from differential expression. All of them were progression-elevated type, and were further certified with Reverse Northern dot blot. Through querying GenBank, two Fragments were highly homologous to the known genes, and three to ESTs with unknown function. |
| Keywords: | Wheat Water stress Differential display |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |