| 猪瘟病毒3′非编码区的多态性及其二级结构分析 |
| |
| 引用本文： | 范运峰,赵启祖,赵耘,邹兴启,张仲秋,王琴,宁宜宝.猪瘟病毒3′非编码区的多态性及其二级结构分析[J].中国农业科学,2010,43(4):828-834. |
| |
| 作者姓名： | 范运峰 赵启祖 赵耘 邹兴启 张仲秋 王琴 宁宜宝 |
| |
| 作者单位： | （中国兽医药品监察所）; |
| |
| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 【目的】分析CSFV3′-UTR一级序列和二级结构,为研究其对病毒复制、增殖和毒力的关系奠定良好的基础。【方法】利用RT-PCR技术扩增CSFV Thiverval株、HCLV株和Shimen株的3′-UTR并测序,使用DNAstar、Sequencher和RNAstructure软件进行CSFV3′-UTR基因组序列的比对分析和二级结构模拟。【结果】Thiverval株3′-UTR最长可达259个碱基,与母源株Alfort相比,含有32nt的插入,最短只有233个碱基,含有6nt的插入。HCLV株最长有244个碱基,含有17nt的插入;最短有233个碱基,含有8nt的插入。同时,疫苗株的插入片段导致3′-UTR空间构型和自由能发生改变,自由能随着插入片段的增加而升高。【结论】(1)CSFV3′-UTR存在两个高度变异的区域。(2)CSFV疫苗株Thiverval株和HCLV株3′-UTR同一样品不同克隆株中插入片段呈现明显的不均一。(3)疫苗株的插入片段影响3′-UTR二级结构的稳定性,可能是导致疫苗株毒力减弱的原因之一。
|
| 关 键 词： | 猪瘟病毒 3′-UTR 不均一性 二级结构分析 |
| 收稿时间： | 2009-04-09; |
Heterogeneity and Secondary Structure Analysis of 3'Untranslated Region in Attenuated Classical Swine Fever Viruses |
| |
| FAN Yun-feng,ZHAO Qi-zu,ZHAO Yun,ZOU Xing-qi,ZHANG Zhong-qiu,WANG Qin,NING Yi-bao.Heterogeneity and Secondary Structure Analysis of 3'Untranslated Region in Attenuated Classical Swine Fever Viruses[J].Scientia Agricultura Sinica,2010,43(4):828-834. |
| |
| Authors: | FAN Yun-feng ZHAO Qi-zu ZHAO Yun ZOU Xing-qi ZHANG Zhong-qiu WANG Qin NING Yi-bao |
| |
| Institution: | FAN Yun-feng1,2,ZHAO Qi-zu1,ZHAO Yun1,ZOU Xing-qi1,ZHANG Zhong-qiu1,WANG Qin1,NING Yi-bao1 (1China Institute of Veterinary Drug Control,Beijing 100081,2China Animal Disease Control Center,Beijing 100125) |
| |
| Abstract: | Objective]The 3'-UTR of CSFV was sequenced and analyzed, for the understanding of the influence of 3'-UTR on replication, multiplication, and virulence of virus.Method]The 3'-UTR of CSFV Thiverval, HCLV and Shimen strains were amplified and sequenced using RT-PCR method.The alignment was carried out using DNAstar and Sequencher software.The RNA secondary structures of different Y-UTR were predicted using RNAstructure software.Result]The longest 3'-UTR of Thiverval strain was 259 base pairs(bp)with a 32 nt insertion.The shortest 3'-UTR had only 233 bp with a 6 nt insertion.The longest Y-UTR of HCLV strain was 244 bp with a 17 nt insertion and the shortest Y-UTR was 235 bp with a 8 nt insertion.At the same time, to maintain the similar Y-UTR structure, the free energy of vaccine strain is higher than that of virulent strain, and the free energy in 3'-UTR of vaccine strain is rising with length of insert.Conclusion]The 3'-UTR of CSFV vaccine strains have two variable regions.The insertion of attenuated Thiverval and HCLV strains Y-UTR represent distinct heterogeneity in different clones of the same sample.The insertion of vaccine strains 3'-UTR could affect the stability of the secondary structure, which might be a reason for the attenuated virulence. |
| |
| Keywords: | 3'-UTR classical swine fever virus 3'-UTR heterogeneity RNA secondary structure |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
