In vivo and in vitro propagation and transmission of Toggenburg orbivirus |
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Authors: | Planzer Jeannette Kaufmann Christian Worwa Gabriella Gavier-Widén Dolores Hofmann Martin A Chaignat Valérie Thür Barbara |
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Institution: | aInstitute of Virology and Immunoprophylaxis, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland;bInstitute of Parasitology, Medical and Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland;cNational Veterinary Institute, 75189 Uppsala, Sweden;dDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, SE-751 89 Uppsala, Sweden |
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Abstract: | The Toggenburg orbivirus (TOV), a recently discovered virus related to bluetongue virus (BTV), has been identified in goats in Switzerland, Italy and Germany. Isolation of TOV in vitro has not yet been achieved and the transmission mechanisms are still unknown. In the experimental infection of pregnant goats described here, TOV could not be detected in secretion/excretion samples or fetal blood. Material from the goat experiment was used as inoculum for propagating the virus in vitro. To enhance the infectivity of TOV several modified protocols, e.g. pretreatment of the virus with trypsin, polyethylene glycol-mediated infection and lipofection were applied. Isolation of TOV, attempts to infect Culicoides nubeculosus by feeding TOV-positive blood and intracerebral inoculation of newborn mice were unsuccessful. The results of these studies suggest that TOV requires specific but different factors than other BTVs for infection and replication outside of its natural caprine host. |
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Keywords: | Toggenburg orbivirus Transmission In vitro isolation Experimental infection Goat |
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