| 植物乳杆菌P158的生长曲线及其细菌素的特性 |
| |
| 引用本文： | 刘书亮,张艾青,田刚,蒲彪,胡欣洁.植物乳杆菌P158的生长曲线及其细菌素的特性[J].核农学报,2009,23(6):1021-1025. |
| |
| 作者姓名： | 刘书亮 张艾青 田刚 蒲彪 胡欣洁 |
| |
| 作者单位： | 1. 四川农业大学食品学院,四川,雅安,625014
2. 四川农业大学动物营养研究所,四川,雅安,625014 |
| |
| 基金项目： | 四川省教育厅重点项目 |
| |
| 摘 要： | 对源于醪糟的植物乳杆菌P158生长曲线分析、药敏实验检测及其细菌素特性的初步研究。结果表明：植物乳杆菌P158培养36h时生物量达到最大值，约为1011 cfu/ml，且培养40h的发酵上清液对藤黄微球菌和铜绿假单胞杆菌的抑菌效果最好，细菌素效价约为1145IU/ml，而发酵上清液pH值降至最低（pH3.53），显示了菌株生长与细菌素的形成密切相关。该菌株对多粘菌素B、多数氨基糖苷类耐药，对青霉素类、四环素类、大环内酯类等药物敏感。细菌素对酸、热稳定，对木瓜蛋白酶、胃蛋白酶、胰蛋白酶、蛋白酶K敏感；对多数的革兰氏阴性菌和革兰氏阳性菌及一些真菌具有较强抑制作用，却对近源乳酸菌几乎无抑制效果。该细菌素可望作为食品生物防腐剂。
|
| 关 键 词： | 乳酸菌 细菌素 植物乳杆菌 生长曲线 特性 |
| 收稿时间： | 2009-05-31 |
Growth Curve of Lactobacillus Plantarum Strain P158 and Its Bacteriocin Characterization |
| |
| LIU Shu-lang,ZHANG Ai-qing,TIAN Gang,PU Biao,HU Xin-jie.Growth Curve of Lactobacillus Plantarum Strain P158 and Its Bacteriocin Characterization[J].Acta Agriculturae Nucleatae Sinica,2009,23(6):1021-1025. |
| |
| Authors: | LIU Shu-lang ZHANG Ai-qing TIAN Gang PU Biao HU Xin-jie |
| |
| Institution: | College of Food ,Sichuan Agriculture University, Ya’an,625014 China;2.Institute of Animal Nutrition ,Sichuan Agriculture University,Ya’an 625014China |
| |
| Abstract: | The characteristics of growth curve and sensitivity to antibiotics of L.plantarum strain P158 selected from fermented glutinous rice and its bacteriocin were studied.The results showed that the maximum biomass was up to 10~(11) cfu/ml at 36 h.The strongest inhibitation activity to Micrococcus luteus and Pseudomonas aerugirnosa and the lowest pH(3.53)were observed after 40 h culture,and titer reached about 1145 IU/ml.It was suggested that the growth of bacteria was closely related to the producing of bacteriocin.The bacteriocin was sensitive to antibiotics,such as penicillins,tetracycline,macrolides and so on,but it Was resistant to polymyxin B and most of aminoglycosides.The bacteriocin was stable in acid and high temperature conditions,but it Was sensitive to trypsin,papain,pepsin and proteinase K.The bacteriocin had broad antimicrobial spectrum including most of gram-negative bacterium and gram-positive bacterium strains,and could inhibit some fungi,however,it almost had no active against LABs.These findings suggest that bacteriocin of strain P158 has the potential as a food biopreservative. |
| |
| Keywords: | lactic acid bacterium bacteriocin Lactobacillus plantarum growth curve characterization |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
