Isolation, characterization, and molecular cloning of cryptic plasmids isolated from Edwardsiella ictaluri |
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| Authors: | J C Newton R C Bird W T Blevins G R Wilt L G Wolfe |
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| Institution: | Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-3501. |
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| Abstract: | Fifty-five isolates of Edwardsiella ictaluri were examined for the presence of plasmid DNA by a rapid alkaline extraction procedure. All 49 isolates from channel catfish and a single isolate from Bengal danio carried 2 plasmids with molecular masses of approximately 3.2 and 3.7 megadaltons (Mdal). Five E ictaluri isolates from other fish contained 1 to 3 plasmids, which had molecular masses ranging from 2.5 to 45 Mdal. The 2 plasmids (3.2 and 3.7 Mdal) from the type strain of E ictaluri (ATCC 33202) were ligated into pUC19 cloning vectors, and restriction endonuclease maps of each insert were prepared. |
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