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| 凤仙花坏死斑病毒的RT-PCR和巢式PCR检测 | |
| 引用本文： | 张巧萍,丁元明,王云月,李旻,周剑,段禄华,白永华,孙兵绍.凤仙花坏死斑病毒的RT-PCR和巢式PCR检测[J].华中农业大学学报,2009,28(1). |
| 作者姓名： | 张巧萍 丁元明 王云月 李旻 周剑 段禄华 白永华 孙兵绍 |
| 作者单位： | 1. 云南出入境检验检疫局,昆明,650228;云南农业大学植物保护学院,昆明,650201 2. 云南出入境检验检疫局,昆明,650228 3. 云南农业大学植物保护学院,昆明,650201 |
| 摘 要： | 根据凤仙花坏死斑病毒(Impatiens necrotic spot tospovirus,INSV)S RNA上的核衣壳蛋白(N)基因序列(登录号为AB109100)保守区设计了3对特异性引物.应用常规RT-PCR和巢式PCR方法从表现同心圆症状的文心兰植株上检测得到了预期DNA片段,且巢式PCR检测灵敏度比常规PCR高.序列分析结果表明,该病毒的N基因序列和已经发表的凤仙花坏死斑病毒(登录号为AB109100、AB207803、DQ425096)核苷酸序列同源性为99%,确定表现同心圆症状的文心兰上携带了凤仙花坏死斑病毒. |
| 关 键 词： | 凤仙花坏死斑病毒 巢式PCR |
Detection of Impatiens necrotic spot tospovirus(INSV)by RT-PCR and Nested PCR |
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| ZHANG Qiao-ping,DING Yuan-ming,WANG Yun-yue,LI Min,ZHOU Jian,DUAN Lu-hu,BAI Yong-hu,SUN Bing-shao.Detection of Impatiens necrotic spot tospovirus(INSV)by RT-PCR and Nested PCR[J].Journal of Huazhong Agricultural University,2009,28(1). | |
| Authors: | ZHANG Qiao-ping DING Yuan-ming WANG Yun-yue LI Min ZHOU Jian DUAN Lu-hu BAI Yong-hu SUN Bing-shao |
| Abstract: | The specific primers were designed based on the conservative region of the N gene of INSV(AB109100).The virus causing chlorotic ringspots on oncidium was identified as Impatiens necrotic spot tospovirus(INSV)through both RT-PCR and the nested PCR amplification technology,which generated a product of the correct predicted size.The sensitivity of the nested PCR is better than normal RT-PCR.The sequencing results showed the homology of N gene sequences between the INSV and the known species is 99%,which confir... |
| Keywords: | RT-PCR |
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