Sperm vitrification of <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: effect of cryoprotectant solutions on sperm viability and spawning quality after artificial insemination |
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| Authors: | Thaís Castelo-Branco Maria Madalena Pessoa Guerra Roberta Soares Silvio Peixoto |
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| Institution: | 1.Departamento de Pesca e Aquicultura,Universidade Federal Rural de Pernambuco,Recife,Brazil;2.Departamento de Medicina Veterinária,Universidade Federal Rural de Pernambuco,Recife,Brazil |
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| Abstract: | Vitrification is a fast freezing method with promising results for penaeids sperm cryopreservation. This study evaluated the efficiency of three cryoprotectant solutions for sperm vitrification and artificial insemination with cryopreserved spermatophores of Litopenaeus vannamei. The cryoprotectant solutions tested were 30% methanol, 2% soy lecithin, and 30% methanol?+?2% soy lecithin. Fully mature females were artificially inseminated with vitrified and fresh spermatophores as a control group. The vitrification method was efficient in maintaining high rates of sperm survival and membrane integrity. Although the egg fertilization was successfully attained by artificial insemination with cryopreserved spermatophores, low hatching rates suggested that possible DNA fragmentation of sperm cells should be further investigated. This is the first report of artificial insemination using vitrified sperm in penaeids. |
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