| 鸭源鸡杆菌对SPF雏鸡的感染特性研究 |
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| 引用本文： | 李乔晶,张九州,杨霞,常洪涛,王珊,皇甫和平,姚惠霞,迪丽拜尔·阿木提,王川庆.鸭源鸡杆菌对SPF雏鸡的感染特性研究[J].中国预防兽医学报,2012,34(5):379-383. |
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| 作者姓名： | 李乔晶 张九州 杨霞 常洪涛 王珊 皇甫和平 姚惠霞 迪丽拜尔·阿木提 王川庆 |
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| 作者单位： | 1. 河南农业大学 牧医工程学院/禽病研究所 动物传染病实验室,河南郑州,450002
2. 新疆阿克苏职业技术学院,新疆阿克苏,843000 |
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| 基金项目： | 中德(Boehringer Ingelheim Vetmedica)合作资助项目(43006167) |
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| 摘 要： | 为研究鸭源鸡杆菌的流行病学特性,本研究采用从自然病例分离得到的鸭源鸡杆菌人工感染4日龄SPF雏鸡,通过形态学、PCR、荧光定量PCR和组织学等方法分别对感染后SPF雏鸡的组织脏器进行了细菌分离、检测和鉴定,用ELISA对其血清抗体水平进行检测.结果表明,人工感染鸡无临床症状,组织学检查感染鸡的肝脏、气管和肺脏组织均有损伤.人工感染后第3d和同居感染第2d鸡体内可分离出鸭源鸡杆菌,人工感染后第96d仍能够分离到鸡杆菌.ELISA方法证实人工感染后47 d和同居后32 d出现抗体高峰,持续2～3周,人工感染组和同居组抗体水平均高于对照组(p＜0.05)；荧光定量PCR检测病料结果显示人工感染组和同居组的气管组织中细菌含量最高.本研究表明雏鸡在4日龄即可感染鸭源鸡杆菌,并能通过同居传播,长期带菌、排菌；感染后抗体产生缓慢、持续期短.本研究为鸭源鸡杆菌的流行病学、致病机理研究及防治措施的制定等提供了参考依据.
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| 关 键 词： | 鸡杆菌 SPF雏鸡 人工感染 同居感染 带菌排菌期 |
Infection and transmission characteristic of Gallibacterium anatis in experimentally infected SPF chicks |
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| LI Qiao-jing
,
ZHANG Jiu-zhou
,
YANG Xia
,
CHANG Hong-tao
,
WANG Shan
,
HUANGFU He-ping
,
YAO Hui-xia
,
DILIBAIER Amuti
,
WANG Chuan-qing.Infection and transmission characteristic of Gallibacterium anatis in experimentally infected SPF chicks[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(5):379-383. |
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| Authors: | LI Qiao-jing ZHANG Jiu-zhou YANG Xia CHANG Hong-tao WANG Shan HUANGFU He-ping YAO Hui-xia DILIBAIER Amuti WANG Chuan-qing |
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| Institution: | 1(1.Animal Infectious Disease Laboratory,Institute of Poultry Diseases,College of Animal Science and Veterinary Medicine, Henan Agricultural University,Zhengzhou 450002,China;2.Job Training College of Xinjiang Akesu,Akesu 843000,China) |
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| Abstract: | To investigate the epidemiological characteristics of Gallibacterium anatis,four day-old SPF chicks were infected with the G.anatis isolated from natural cases.Bacteria in organs of the SPF chicks were detected and identified post infection by the morphology,PCR,fluorescence quantitative PCR and histology,respectively,and serum antibody tiers were detected by ELISA.The results showed that no clinical signs were observed in experimentally infected birds,however,histopathologic examinations of liver,lung and trachea revealed lesions in all infected chickens.G.anaits was isolated and identified from SPF chicks 3 days post infection(p.i.) and 2 days post cohabiting infection,and it still was able to detect at 96 days p.i.ELISA detection results showed that the peak titers of antibodies in infected birds and cohabiting birds appeared at 47 and 32 days,respectively,which lasted for 2 to 3 weeks.Results of G.anaits detection in organs by fluorescence quantitative PCR showed that bacteria in tracheal from infected birds and cohabiting birds were the highest.This study showed that chicks could be infected with G.anatis as early as 4 day-old and the bacteria spread via cohabiting,long-term carrying and shedding.Antibody was produced slowly and the duration of peak was short after infection.This study provided a reference basis on epidemiology,pathogenesis research and prevention of G.anatis. |
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| Keywords: | Gallibacterium SPF chicks experimental infection cohabiting infection excrete periods law of producing antibody |
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