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不同类型东北梅花鹿亲缘关系的研究
引用本文:高志光,岳秉飞,马朝红.不同类型东北梅花鹿亲缘关系的研究[J].延边大学农学学报,2009,31(1):1-5.
作者姓名:高志光  岳秉飞  马朝红
作者单位:1. 北华大学,吉林,吉林,132011
2. 中国药品鉴定所,北京,100094
3. 双辽市农业局,吉林,双辽,132109
基金项目:科技部国家科技资源平台项目 
摘    要:用聚丙烯酰胺凝胶电泳法测定140头东北梅花鹿5个类型的转铁蛋白(Tf)的多态性.Tf有6种表型,受TfA1、TfA2、TfB1、TfB2、TfC1、TfC2等6个复等位基因控制.比较梅花鹿5种类型间Tf基因频率,类型间有较大差异.利用同质度(H·I)、平均杂和性(H)和有效基因数(Ne)比较5种类型间的遗传变异性,双阳型和伊通型变异性较大,其次是东丰型、抚松型和龙潭型.根据基因频率计算遗传距离,并用类平均法进行聚类分析,结果表明,东丰型与抚松型、双阳型与伊通型的亲缘关系较近,龙潭型与其它4种类型有较大的分化.

关 键 词:东北梅花鹿  转铁蛋白  电泳  类型

Study of sibship of Cervus hortulorum
GAO Zhi-guang,YUE Bing-fei,LI Sheng.Study of sibship of Cervus hortulorum[J].Journal of Agricultural Science Yanbian University,2009,31(1):1-5.
Authors:GAO Zhi-guang  YUE Bing-fei  LI Sheng
Institution:GAO Zhi-guang , YUE Bing-fei , LI Sheng(1. Beihua University , Jilin Jilin 132011, China 2. Medicine and Chemical Reagents Identification Institute of China ,Beijing 100094, China Special Product Institute of Chinese Academy of Agricultural Sciences, Jilin Jilin 132109,China)
Abstract:The Transferrin(Tf)polymorphism of 5 types for 140 heads of Cervus hortulorum was measured by the way of polyacrylamide gelelectrophoresis. The six phenptypes of Tf were controlled by six compound allelic genes, which were TfA1, TfA2, TfB1, TfB2, TfC1, and TfC2. There were instinct differences between five types in gene frequency. Genetic varlability of Shuangyang and Yitong were more larger than Dongfeng,Fusong and Longtan by the means of comparing genetic variability of H ·I, average heterozygosity and effective gene dosage. Genetic distance was calculated with gene efficiency and cluster analysis was made by cluster average method. The results showed that the genetic relationships of Dong-feng and Fusong, Shuangyang and Yitong were close, and the differentiation of Longtan and the other four types above was greater.
Keywords:Cervus hortulorun  greater  transferrin  electrophoresis  type
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