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| 基于Gateway技术的拟南芥雄性不育GHF 基因表达载体的构建 | |
| 引用本文： | 胡金艳,张书祥,王晓武,武剑.基于Gateway技术的拟南芥雄性不育GHF 基因表达载体的构建[J].中国农学通报,2007,23(12). |
| 作者姓名： | 胡金艳 张书祥 王晓武 武剑 |
| 作者单位： | 1. 安徽大学生命科学院,合肥,232007;中国农业科学院蔬菜花卉研究所,北京,100081 2. 安徽大学生命科学院,合肥,232007 3. 中国农业科学院蔬菜花卉研究所,北京,100081 |
| 摘 要： | 随着模式植物拟南芥全基因组测序工作的完成更多雄性不育相关基因功能需要阐明。利用拟南芥突变体库,从反向遗传学角度进行突变基因的功能研究需要构建相应的载体进行互补实验。Gateway技术是一种高效,快速的克隆系统。笔者利用Gateway克隆技术成功构建拟南芥GHF基因表达载体,并遗传转化拟南芥,为进一步阐明该基因功能奠定了基础。 |
| 关 键 词： | Gateway克隆技术 拟南芥(Arabidopsis thaliana) 雄性不育 GHF基因 表达载体 |
Construction of Expression Vector of Arabidopsis Male Sterile GHF Gene Using Gateway Cloning Technology |
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| Hu Jinyan,Zhang Shuxiang,Wang Xiaowu,Wu Jian.Construction of Expression Vector of Arabidopsis Male Sterile GHF Gene Using Gateway Cloning Technology[J].Chinese Agricultural Science Bulletin,2007,23(12). | |
| Authors: | Hu Jinyan Zhang Shuxiang Wang Xiaowu Wu Jian |
| Abstract: | With the completion of the whole genome sequence of mode plant Arabidopsis, more functions of male sterile gene need to be expounded. Using Arabidopsis mutant bank to identify functions of mutant gene and corresponding vector need to be constructed .In order to carry out the complementary experiment. Gateway cloning technology is a fast cloning system. In this article, using Gateway technology GHF gene expression vector was constructed and introduced into Arabidopsis. It was useful for further gene functional research. |
| Keywords: | Gateway cloning technology Arabidopsis thaliana male sterile GHF gene expression vector |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |