A coagglutination assay with monoclonal antibodies for rapid laboratory identification of Mycoplasma synoviae. |
| |
| Authors: | M A Morsy V S Panangala M M Gresham M Toivio-Kinnucan |
| |
| Institution: | Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama. |
| |
| Abstract: | An agglutinating monoclonal antibody (MAb S2) specific for a 55,000-molecular-weight surface protein of Mycoplasma synoviae was developed by fusion of spleen cells from immunized BALB/c mice with P3X63 Ag8.653 myeloma cells. Immunogold labeling experiments confirmed the cell surface location of the MAb-recognized antigen. MAb S2-coated Staphylococcus aureus was used in a rapid slide coagglutination assay. Eleven M. synoviae strains, including the type strain WVU 1853, coagglutinated with MAb-coated S. aureus, but five M. gallisepticum strains (PG31, S6, R, F, and A5969) did not. |
| |
| Keywords: | |
|
|
