| Cu2+, Zn2+和Mn2+对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响 |
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| 引用本文： | 李涛,于贤昌.Cu2+, Zn2+和Mn2+对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响[J].园艺学报,2007,34(4):895-900. |
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| 作者姓名： | 李涛 于贤昌 |
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| 作者单位： | 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金;山东省自然科学基金 |
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| 摘 要： | 以自根和嫁接黄瓜幼苗为试材,研究叶片Mn、Cu、Zn和Fe含量及低温胁迫下SOD活性的差异,结果表明:嫁接黄瓜叶片Mn、Cu和Zn的含量均显著高于自根黄瓜,而Fe的含量则显著低于自根黄瓜;低温胁迫第3天,嫁接黄瓜叶片SOD、Cu/Zn-SOD和Mn-SOD活性均显著高于自根黄瓜,Fe-SOD则显著低于自根黄瓜。为探索嫁接黄瓜叶片高含量Mn、Cu、Zn与SOD活性之间的关系,向低温胁迫第3天的自根黄瓜叶片SOD提取液中加入Mn2 、Cu2 和Zn2 ,使之与嫁接黄瓜叶片SOD提取液对应组合的Mn2 、Cu2 和Zn2 浓度相等,研究此时的SOD及其同工酶活性的变化,结果发现:Mn2 、Cu2 和Zn2 加入自根黄瓜叶片提取液后均能显著增强SOD活性,其增强作用的大小依次为Mn2 Zn2 ,Mn2 Cu2 ,Mn2 ,Cu2 ,Cu2 Zn2 Mn2 ,Cu2 Zn2 和Zn2 。3种离子的加入都不影响Fe-SOD的活性。这一结果说明黄瓜叶片Mn、Cu和Zn的含量是影响SOD活性大小的重要因素,嫁接黄瓜吸收Mn、Cu和Zn的能力较强是其SOD活性显著高于自根黄瓜的重要原因之一。
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| 关 键 词： | 黄瓜 嫁接 微量元素 低温胁迫 SOD活性 |
| 文章编号： | 0513-353X（2007）04-0895-06 |
| 修稿时间： | 2007-04-26 |
Effect of Cu2+, Zn2+and Mn2+on SOD Activity of Cucumber Leaves Extraction after Low Temperature Stress |
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| LI Tao,YU Xian-chang.Effect of Cu2+, Zn2+and Mn2+on SOD Activity of Cucumber Leaves Extraction after Low Temperature Stress[J].Acta Horticulturae Sinica,2007,34(4):895-900. |
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| Authors: | LI Tao YU Xian-chang |
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| Abstract: | The contents of Mn, Zn, Cu, Fe and the activities of superoxide dismutase (SOD) and its isoenzymes in grafted and own-rooted cucumber leaves under low temperature stress were studied. The results indicated that the contents of Mn, Cu, Zn and the activities of SOD, Cu/Zn-SOD and Mn-SOD in grafted cucumber leaves were significantly higher than those in own-rooted cucumber leaves, while the content of Fe and Fe-SOD activity was lower. In order to make clear the relationships between high contents of Mn, Zn, Cu and SOD activity in grafted cucumber, Mn2 ,Zn2 and Cu2 and different combinations were added into own-rooted cucumber leaves extraction, respectively, in the third day during low temperature stress and made their final concentrations were the same as they in grafted cucumber leaves extraction. The results showed that adding Mn2 ,Zn2 , Cu2 and their different combinations were added into own-rooted cucumber leaves extraction could enhance the SOD and its isoenzymes activities except the Fe-SOD. The sequence of increasing level was Mn2 Zn2 , Mn2 Cu2 , Mn2 , Cu2 , Cu2 Zn2 Mn2 , Cu2 Zn2 and Zn2 .This research has proved that the Mn, Cu and Zn in cucumber leave could affect the SOD activity and the higher SOD activity in grafted cucumber leave might be attributed to higher accumulation of Cu, Zn and Mn. |
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| Keywords: | Cucumber Graft Microelement Low temperature stress SOD activity |
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