| 绵羊regakine-1基因的克隆与序列分析 |
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| 引用本文： | 乔新安,杨国宇,王艳玲,王月影,韩立强,都军霞.绵羊regakine-1基因的克隆与序列分析[J].中国农学通报,2007,23(8):40-40. |
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| 作者姓名： | 乔新安 杨国宇 王艳玲 王月影 韩立强 都军霞 |
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| 作者单位： | 河南农业大学动物生理生化实验室,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目＂乳汁体细胞分子生物学技术平台的建立与应用＂（0523010500）. |
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| 摘 要： | 【研究目的】克隆并分析绵羊regakine-1基因；【方法】肠系膜淋巴结组织提取总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD-19T载体连接后转化JM109感受态细胞，筛选阳性克隆、测序，并进行序列分析；【结果】克隆的绵羊regakine-1基因与牛的同源性为91%，推测的氨基酸序列信号肽为1~21aa，SCY结构域为29~87aa，结构特征与牛的相一致。【结论】克隆了绵羊regakine-1基因的ORF，并注册GenBank (AccessionEF617337)
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| 关 键 词： | 陕北农牧交错带 陕北农牧交错带 粮食安全 植物性产品 动物性产品 |
| 修稿时间： | 2007-05-262007-06-07 |
Molecular Cloning and Sequence Analysis of Ovine Regakine-1 |
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| Qiao Xinan,Yang Guoyu,Wang Yanling,Wang Yueying,Han Liqiang,Du Junxia.Molecular Cloning and Sequence Analysis of Ovine Regakine-1[J].Chinese Agricultural Science Bulletin,2007,23(8):40-40. |
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| Authors: | Qiao Xinan Yang Guoyu Wang Yanling Wang Yueying Han Liqiang Du Junxia |
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| Institution: | (Lab of Animal Physiology & Biochemistry Henan Agricultural University, Zhengzhou 450002) |
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| Abstract: | OBJECTIVE]To clone and analysis the ovine regakine-1.METHOD]Total RNA was extracted from mesenteric lymph node and mRNA sequence of gene were amplified by RT-PCR with designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The recombinant plasmid was identified and the sequence was analyzed.RESULTS]Identity analysis showed that the regakine-1 nucleotide sequence shared 91% homology with that of cattle. The signal peptide of predicted amino acid sequence is 1~21aa and the structural domain SCY is 29~87aa, revealing that the structural feature is consistent with cattle regakine-1.CONCHUSION]The ovine ORF of regakine-1 gene was successfully cloned in present study and the sequence has been submitted to GenBank(Accession. EF617337). |
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| Keywords: | Ovine Regakine-1 Molecular Cloning Sequence Analysis |
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