| 哈茨木霉T2-16突变体库的构建及突变株表型分析 |
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| 引用本文： | 何英,张屹,肖姬玲,朱菲莹,魏林,梁志怀.哈茨木霉T2-16突变体库的构建及突变株表型分析[J].安徽农业大学学报,2019,46(6):1034-1039. |
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| 作者姓名： | 何英 张屹 肖姬玲 朱菲莹 魏林 梁志怀 |
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| 作者单位： | 湖南大学研究生院隆平分院,长沙410125;湖南省农业生物技术研究所,长沙410125;湖南省植物保护研究所,长沙410125;湖南大学研究生院隆平分院,长沙410125;湖南省农业生物技术研究所,长沙410125 |
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| 基金项目： | 湖南农业科技创新项目（2017JC77）, 国家重点研发计划( 2017YFD0200606)和国家公益性行业(农业)科研专项（201503110-03）共同资助。 |
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| 摘 要： | 为了优化高效拮抗生防菌哈茨木霉T2-16的EMS诱变条件，构建哈茨木霉T2-16突变体库，为哈茨木霉功能基因组研究奠定材料基础，以哈茨木霉T2-16为试验材料，采用单因素试验以0～0.6 mol·L-1 EMS溶液分别处理不同浓度孢子0～6 h，明确适于哈茨木霉诱变的EMS最佳浓度、处理时间和孢子浓度。以最适参数诱变处理野生型菌株，进行生长势、拮抗能力和生防效果比较。结果显示，0.4 mol·L-1 EMS溶液处理浓度为106 ·mL-1的孢子4 h，其致死率接近70%，诱变效果最佳。经生长势、拮抗能力和生防效果比较发现，生长势呈正向突变占有率达46.20%，其中，16株突变菌株拮抗作用明显增强，T42和T62的生防效果分别高出野生型菌株31.64%和27.91%，为研究哈茨木霉与生防相关的功能基因打下基础。
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| 关 键 词： | 哈茨木霉T2-16 EMS诱变 突变体库 表型分析 |
Construction of Trichoderma harzianum T2-16 mutant library and phenotypic analysis of mutant |
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| HE Ying,ZHANG Yi,XIAO Jiling,ZHU Feiying,WEI Lin and LIANG Zhihuai.Construction of Trichoderma harzianum T2-16 mutant library and phenotypic analysis of mutant[J].Journal of Anhui Agricultural University,2019,46(6):1034-1039. |
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| Authors: | HE Ying ZHANG Yi XIAO Jiling ZHU Feiying WEI Lin and LIANG Zhihuai |
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| Institution: | Longping Branch, Graduate School of Hunan University, Changsha 410125,Hunan Agricultural Biotechnology Research Institute, Changsha 410125,Hunan Agricultural Biotechnology Research Institute, Changsha 410125,Hunan Agricultural Biotechnology Research Institute, Changsha 410125,Institute of Plant Protection of Hunan Province, Changsha 410125 and Longping Branch, Graduate School of Hunan University, Changsha 410125; Hunan Agricultural Biotechnology Research Institute, Changsha 410125 |
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| Abstract: | To optimize the EMS mutagenesis conditions of Trichoderma harzianum T2-16, we constructed a mutant library of T. harzianum T2-16 to lay a material foundation for the study of the functional genome of T. harzianum. Using T. harzianum T2-16 as the experimental material, the spores were treated with 0-0.6 mol·L-1 EMS solution for 0-6 h, respectively. The optimum concentration, treatment time and spore concentration of EMS mutagenesis were determined. The wild-type strains were mutagenized with the optimum parameters, and the growth potential, antagonistic ability and biocontrol effect were compared. The results showed that spores treated with 0.4 mol·L-1 EMS solution at 106 ·mL-1 spore concentration for 4 h had the best mutagenic effect, with the lethal rate of about 70%. By comparing growth potential, antagonistic ability and biocontrol effect, it was found that the positive mutation rate in growth potential was 46.20%. Among them, the antagonistic effects of 16 mutant strains were significantly enhanced. The biocontrol effect of T42 and T62 was 31.64% and 27.91% higher than that of wild type strains, respectively. |
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| Keywords: | Trichoderma harzianum T2-16 EMS mutagenesis mutant library phenotypic analysis |
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