| 甜瓜POD同工酶及其Fuzzy聚类分析 |
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| 引用本文： | 郭素枝,何承坤,李家慎,蔡天清.甜瓜POD同工酶及其Fuzzy聚类分析[J].福建农林大学学报(自然科学版),1992(3). |
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| 作者姓名： | 郭素枝 何承坤 李家慎 蔡天清 |
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| 作者单位： | 福建农学院园艺系，福建农学院园艺系，福建农学院园艺系，福建农学院园艺系 福州 350002，福州 350002，福州 350002，福州 350002 |
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| 摘 要： | 采用PAGE、酶谱紫外扫描及Fuzzy聚类法研究薄皮甜瓜与厚皮甜瓜功能叶POD同工酶的结果表明,甜瓜POD同工酶共有11条正极带,其中的3条是薄皮甜瓜与厚皮甜瓜的基础酶带.供试薄皮甜瓜各品种POD同工酶均为6条酶带,其中2条为该类群的特征酶带.供试厚皮甜瓜各品种POD同工酶均为9条酶带,其中3条为该类群的特征酶带.Fuzzy聚类分析表明,两类甜瓜品种间POD同工酶谱的相似系数为(0.50,1.00),其隶属度为(0.64,1.00),厚皮甜瓜品种含笑与薄皮甜瓜的亲缘关系比与厚皮甜瓜其它品种的亲缘关系更近,厚皮甜瓜与薄皮甜瓜在分子水平上没有截然的界限.从甜瓜POD同工酶角度证明了两类甜瓜具有初生起源中心及次生起源中心的分化.甜瓜感染花叶病后,植株的POD同工酶活性增强,某些品种出现了新酶带.
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| 关 键 词： | 甜瓜 POD同工酶 模糊聚类 病毒病 |
Peroxidase Isozyme in Cucumis melo L. and Its Fuzzy Cluster Analysis |
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| Guo Suzhi He Chengkun Li Jiashen Cai Tianqing.Peroxidase Isozyme in Cucumis melo L. and Its Fuzzy Cluster Analysis[J].Journal of Fujian Agricultural and Forestry University,1992(3). |
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| Authors: | Guo Suzhi He Chengkun Li Jiashen Cai Tianqing |
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| Abstract: | The peroxidase isozyme of the funtional leaves of 8 cultivars in Cucumis melo L. was assayed by using polyacrylasmide gel electrophoresis, isozyme—grams scanning and fuzzy cluster analysis. The results indicated that there were 11 positive bands of peroxidase isozyme in oriental melon and cantaloupe, and 3 of them were basic bands; Cantaloupe had 9 iso-bands, and 3 of them were characteristic bands; Oriental melon 6 iso-bands, and 2 of them characteristic bands. The results of fuzzy cluster analysis showed that the relationships between cantaloupe Hanxiao and all of oriental melon cultivars were much closer than those between cantaloupe Hanxiao＇ and the other cultivars of cantaloupe, which indicated that two horticultural groups of Cucumis melo L., i. e. oriental melon and cantaloupe, could not be distinguished in molecular level. The facts that oriental melon and cantaloupe had commonly primary center of origin and the differentiation of secondary center of origin were verified by POD isozyme. Increased new bands in some cultivars and activities in POD isozyme were found on the plants infected by mosaic disease. |
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| Keywords: | Cucumis melo L POD isozyme Fuzzy cluster virus disease |
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