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| 牛日本血吸虫病5种血清学诊断技术比较 | |
| 引用本文： | 向静,刘毅,江为民,曹满湖,李娜.牛日本血吸虫病5种血清学诊断技术比较[J].湖南农业大学学报(自然科学版),2007,33(1):49-52. |
| 作者姓名： | 向静 刘毅 江为民 曹满湖 李娜 |
| 作者单位： | 1. 长沙学院,生物工程与环境科学系,湖南,长沙,410003 2. 湖南农业大学,动物医学院,湖南,长沙,410128 3. 湖南省畜牧水产局,技术推广站,湖南,长沙,410006 |
| 基金项目： | 湖南省科学技术厅项目(2006SK1001) |
| 摘 要： | 为找寻特异性强、敏感性高,且操作简便、费用低廉,并能大规模应用于临床的日本血吸虫病血清学诊断方法,以疫区黄牛为研究对象,应用环卵沉淀试验(COPT)、血清法间接血凝试验(sIHA)和血纸法间接血凝试验(bpIHA)、胶乳凝集试验(PAPS)、三联斑点酶联免疫吸附试验(三联Dot-ELISA)5种血清学诊断方法与粪检方法检测牛日本血吸虫病,并对5种血清学检测结果进行评价.检测分析结果表明,用血清学方法COPT,sIHA,三联Dot-ELISA对疫区黄牛进行血吸虫病检测,与三粪九检结果符合率高,具有灵敏度高、特异性好、简捷方便、费用低等特点,特别适用于疫区家畜血吸虫病的诊断、普查,是比较理想的现场检测方法,值得推广.血清学方法bpIHA和PAPS检测血吸虫病,尽管方法简便、费用低廉,但其灵敏度、特异性与三粪九检结果符合率低于血清学方法COPT,sIHA,三联Dot-ELISA,易造成漏检,假阳性,故在普查时,不建议采用. |
| 关 键 词： | 黄牛 日本血吸虫病 粪检 血清学诊断 |
| 文章编号： | 1007-1032(2007)01-0049-04 |
| 修稿时间： | 2006年3月6日 |
Comparison on five kinds of serological diagnose assays of Schistosomiasis japonicum in farm cattle |
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| XIANG Jing,LIU Yi,JIANG Wei-min,CAO Man-hu,LI Na.Comparison on five kinds of serological diagnose assays of Schistosomiasis japonicum in farm cattle[J].Journal of Hunan Agricultural University,2007,33(1):49-52. | |
| Authors: | XIANG Jing LIU Yi JIANG Wei-min CAO Man-hu LI Na |
| Abstract: | To find an optimal of diagnostic method,which are supposed to be cheap,higher sensitive,specific,easy in preparation,and applicable in field diagnosis,stool exam and five kinds of serological detective methods such as COPT,sIHA,bpIHA,PAPS,Three-united-Dot-ELISA were employed detect the Schistosomiasis japonicum.Using the stool exam as the contrast to evaluate effectiveness of the five methods,the result showed that the test result of COPT,sIHA and three-united-Dot-ELISA were according with stool exam,which is sensitive,special,easier and cheaper.They were optimum method to exam Schistosomiasis japonicum in farm.While,the results suggested that the bpIHA and PAPS were not effective. |
| Keywords: | farm cattle Schistosomiasis japonicum stool exam diagnosis of serology |
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