| MDCC—MSB1培养基上清液中DNA活性的定性研究 |
| |
| 引用本文： | 李建伟,马云燕.MDCC—MSB1培养基上清液中DNA活性的定性研究[J].中国预防兽医学报,1999,21(5):379-381. |
| |
| 作者姓名： | 李建伟 马云燕 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所!哈尔滨150001(李建伟，马云燕，白侠，李曦，徐宜为)，中国农业科学院哈尔滨兽医研究所!哈尔(刘宝全) |
| |
| 摘 要： | 将 Ｍ Ｄ Ｃ Ｃ Ｍ Ｓ Ｂ１ ４８ 小时培养物 １０００ｒ／ｍ ｉｎ 离心的上清液分别用 Ｒ Ｎ Ａ 酶、 Ｄ Ｎ Ａ 酶和蛋白酶 Ｋ 处理后，进行体外试验。结果表明，只有 Ｄ Ｎ Ａ 酶处理后的上清液失去了体外抑制 Ｍ Ｄ Ｖ“８１４”增殖的作用。将该上清液 １００００ｒ／ｍ ｉｎ 离心所得的沉淀分别用上述酶处理后进行体内试验。结果表明，只有 Ｄ Ｎ Ａ 酶处理的样品失去了体内促进 Ｍ Ｄ Ｖ 京１ 株致瘤的作用。同时，电泳分析结果证明，该上清液中确实存在 Ｄ Ｎ Ａ。
|
| 关 键 词： | MDCC-MSB1 培养物上清液 MDV DNA |
Determination on the Action of DNA in Cell free MDCC MSB1 Culture Supernatant to Marek's Disease in vitro and vivo |
| |
| LI Jianwei,MA Yunyan,BAI Xai,LI Xi,XU Yiwei,LUI Baoquan.Determination on the Action of DNA in Cell free MDCC MSB1 Culture Supernatant to Marek''s Disease in vitro and vivo[J].Chinese Journal of Preventive Veterinary Medicine,1999,21(5):379-381. |
| |
| Authors: | LI Jianwei MA Yunyan BAI Xai LI Xi XU Yiwei LUI Baoquan |
| |
| Abstract: | We have demonstrated the cell free MDCC MSB1 culture supernatant could inhibit the Marek's disease virus(MDV)propagation on CEF in vitro while promote oncogenicity of Jing 1 MDV challenged Chicken in vitro.In order to investigate which component playing the functional roles,1000 rpm centrifugated cell free MDCC MSB1 Culture supernatants were digested with RNase,DNase and proteinase K respectively and used to inoculate CEF culture together with MDV'814'.Thase above cell free supernatants were also centrifugated by 10000 rpm.The collected sediments were digested with Rnase,Dnase and proteinase K respectively too,and then used to inoculate SPF chickens together with Jing 1 MDV challenge.The results revealed that only the Dnase digested cell free supernatants and sediments respectively lost the inhibition effects of MDV propagation in vitro and the promotion effects of oncogenicity in vitro.The existence of DNA in the cell free supernatant was verified by gel electrophoresis analysis. |
| |
| Keywords: | MDCC MSB1 Culture supernatant Marek's disease virus DNA |
| 本文献已被 CNKI 维普 等数据库收录！ |
