| 大肠杆菌肠毒素ST1—LTB融合蛋白工程菌株的免疫原性研究 |
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| 引用本文： | 许崇波,卫广森.大肠杆菌肠毒素ST1—LTB融合蛋白工程菌株的免疫原性研究[J].中国预防兽医学报,1999,21(1):43-45. |
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| 作者姓名： | 许崇波 卫广森 |
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| 作者单位： | 解放军农牧大学军事兽医研究所 |
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| 摘 要： | 已构建的能表达大肠杆菌肠毒素ＳＴ１ＬＴＢ融合蛋白的工程菌株ＢＬ２１（ＤＥ３）（ｐＸＥＴＳＬＴ１）及其表达产物经动物试验证实没有毒性反应。用从ＩＰＴＧ诱导的工程菌中提取的包涵体或经甲醛灭活的工程菌制成抗原，免疫小鼠，结果免疫小鼠至少能抵抗１５ＭＬＤ的大肠杆菌强毒株Ｃ８３９０２（Ｋ８８ａｃ，ＳＴ＋，ＬＴ＋）的攻击。用提取的包涵体免疫家兔后，采集的血清能够中和天然ＳＴ１的毒性。这表明构建的工程菌株ＢＬ２１（ＤＥ３）（ｐＸＥＴＳＬＴ１）可以作为预防幼畜大肠杆菌性腹泻基因工程菌苗的候选株。
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| 关 键 词： | 大肠杆菌 ST_1 LT_B |
Immunogenicity of the Genetic Engineering Strain Expressing Escherichia coli Heat stable Enterotoxin I and Heat labile Enterotoxin B Subunit Fusion Protein |
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| XU chongbo,WEI Guangsen,WANG Zhuo et al.Immunogenicity of the Genetic Engineering Strain Expressing Escherichia coli Heat stable Enterotoxin I and Heat labile Enterotoxin B Subunit Fusion Protein[J].Chinese Journal of Preventive Veterinary Medicine,1999,21(1):43-45. |
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| Authors: | XU chongbo WEI Guangsen WANG Zhuo |
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| Abstract: | Fusion protein of heat stable enterotoxin I and heatlabile enterotoxin B subunit of Escherichia coli was expressed in recombinant strain BL21(DE3)(pXETSLT1),and completely lost its activity of the toxin.Mice immunized with crude inclusion bodies containing ST 1 LT B fusion protein or inactivated recombinant strain produced antibodies that were able to provide protection in a mouse model against the challenge of at least 1 5MLD dose of Escherichia coli C83902.Importantly,rabbit immunized with crude inclusion bodies containing ST 1 LT B fusion protein could produce antibodies that were able to neutralize the biological activity of native ST 1 enterotoxin in the suckling mouse assay.Hence the recombinant strain BL21(DE3)(pXETSLT1)can be used as a candidate of vaccine strain. |
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| Keywords: | Escherichia coli Heat stable enterotoxin I Heat labile enterotoxin B subunit |
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