Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells |
| |
| Authors: | C E Hart C Y Ou J C Galphin J Moore L T Bacheler J J Wasmuth S R Petteway G Schochetman |
| |
| Institution: | Centers for Disease Control, Atlanta, GA 30333. |
| |
| Abstract: | Host cell factors act together with regulatory genes of the human immunodeficiency virus (HIV) to control virus production. Human-Chinese hamster ovary hybrid cell clones were used to probe for human chromosomes involved in regulating HIV gene expression. DNA transfection experiments showed that 4 of 18 clones had high levels of HIV gene expression measured by both extracellular virus production and transactivation of the HIV long terminal repeat in the presence of the trans-activator (tat) gene. Karyotype analyses revealed a 94% concordance (17/18) between human chromosome 12 and HIV gene expression. Other chromosomes had an 11 to 72% concordance with virus production. |
| |
| Keywords: | |
|
