Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA |
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| Authors: | Andersen Christian B F Ballut Lionel Johansen Jesper S Chamieh Hala Nielsen Klaus H Oliveira Cristiano L P Pedersen Jan Skov Séraphin Bertrand Le Hir Hervé Andersen Gregers Rom |
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| Institution: | Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark. |
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| Abstract: | In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I. |
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