| 沙门菌19个毒力岛标志性基因的PCR检测 |
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| 引用本文： | 胡兴娟,沈飚,王忠发,杨赛军,徐君辉.沙门菌19个毒力岛标志性基因的PCR检测[J].动物医学进展,2016(5):20-25. |
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| 作者姓名： | 胡兴娟 沈飚 王忠发 杨赛军 徐君辉 |
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| 作者单位： | 舟山出入境检验检疫局,浙江舟山,316021 |
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| 基金项目： | 国家质检总局科技计划项目(2014IK278)；浙江省分析测试科技计划项目(2014C37108) |
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| 摘 要： | 建立沙门菌19个毒力岛标志性基因检测方法,了解沙门菌中毒力岛的分布情况。通过比对验证挑选出45个毒力基因作为19个毒力岛标志性基因,建立PCR检测方法,并对伤寒、肠炎、鼠伤寒及贝坎道夫沙门菌进行分布情况研究。19个毒力岛上45个标志性基因分别在伤寒、肠炎、鼠伤寒及贝坎道夫沙门菌得到验证,PCR扩增产物与预期扩增产物一致,电泳条带典型,无非特异扩增条带及杂带出现。45个标志性基因在伤寒、肠炎、鼠伤寒及贝坎道夫沙门菌检出率分别为75.56%、75.56%、66.67%和42.22%。本研究建立的45个毒力岛标志性基因检测方法具有结果准确、简单快速、费用低廉等优点,适用于沙门菌毒力岛研究及不同来源的沙门菌致病性风险评估。
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| 关 键 词： | 沙门菌 毒力岛 标志性基因 PCR 检测 |
PCR Detection of Iconic Genes in 19 Salmonella Pathogenicity Islands |
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| Abstract: | To develop a PCR method for detection of Salmonella pathogenicity island genes and to investi-gate the distribution of pathogenicity islands in Salmonella,by comparing selected 45 virulence genes as the iconic genes of 19 pathogenicity islands,PCR assay was established for detecting S.typhi,S.enteritidis,S. typhimurium and S.bergedorf.45 iconic virulence genes distributed among the 19 pathogenicity islands were validated in S.typhi,S.enteritidis,S.typhimurium and S.bergedorf.The length of amplified prod-ucts were the same as expected and bands of which were rather typical and clear.The detection rates of 45 virulence genes were 75.56%,75.56%,66.67% and 42.22%,respectively,in S.typhi,S.enteritidis,S. typhimurium and S.bergedorf.The PCR method which was accurate,rapid and low-cost was available for studying Salmonella pathogenicity islands and evaluating the relative risk. |
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| Keywords: | Salmonella pathogenicity island iconic gene PCR detection |
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