| 维生素C对红细胞氧化损伤保护作用研究 |
| |
| 引用本文： | 李嘉欣,钱多,解彩霞,苏燕.维生素C对红细胞氧化损伤保护作用研究[J].动物医学进展,2016(5):60-62. |
| |
| 作者姓名： | 李嘉欣 钱多 解彩霞 苏燕 |
| |
| 作者单位： | 1. 包头医学院生物化学与分子生物学教研室,内蒙古包头,014060;2. 包头市第四医院检验科,内蒙古包头,014030 |
| |
| 基金项目： | 国家自然科学基金项目(81160214)；内蒙古自然科学基金项目(2010BS1101)；内蒙古自治区高等学校科学研究项目(NJZY14265)；秦文斌科技教育基金项目(201309)；内蒙古卫生厅科技计划项目(2010056) |
| |
| 摘 要： | 为了利用血红蛋白释放试验(HRT)检测生理浓度维生素C(VC)对生理浓度过氧化氢(H2O2)氧化损伤后红细胞的保护作用,用终浓度为0、0.15、0.3、0.4、0.6、0.7、0.85mmol/L的VC溶液处理红细胞悬液并进行HRT。再分别用0、1.25、2.50、3.75、5.00mL/L的H2O2溶液处理0.4mmol/L VC作用后红细胞悬液(VC保护组)和VC未作用的红细胞悬液(对照组),测定红细胞相对溶解度。结果表明,VC达到生理浓度时,血红蛋白再释放量逐渐减弱;VC保护组红细胞相对溶解度与对照组差别不大。说明生理浓度的VC能减弱HRT的血红蛋白再释放,降低红细胞破膜率,但不能显著降低H2O2对细胞膜的氧化损伤。
|
| 关 键 词： | 维生素 C 血红蛋白释放试验 过氧化氢 氧化损伤 |
Protective Effect of Vitamin C on Oxidative Damage of Erythrocytes |
| |
| Abstract: | In order to use hemoglobin releasing test (HRT)to detect the protective effect of physiological concentration of vitamin C on erythrocytes treated by physiological concentration of hydrogen peroxide (H2 O2 ),The final concentrations of 0,0.15,0.3,0.4,0.6,0.7,0.85 mmol/L vitamin C solution,were used to treat erythrocyte suspensions and HRT was conducted.Then 0,1.25,2.50,3.75,5.00 mL/L H2 O2 solutions were used to treat 0.4mmol/L vitamin C treated erythrocyte suspension (vitamin C protec-tion groups)and vitamin C untreated erythrocyte suspension (control groups)respectively,and the relative solubility of erythrocytes was measured.The results showed that the release of hemoglobin decreased grad-ually when the concentration of vitamin C reached to the physiological level.The relative solubility of e-rythrocytes in vitamin C protection groups had no significant difference from that of the control groups.It showed that the physiological concentration of vitamin C can decrease the release of hemoglobin by HRT, and decrease the rate of erythrocyte membrane breaking rate,but it can not significantly reduce the oxida-tive damage of H2 O2 on the cell membrane. |
| |
| Keywords: | vitamin C hemoglobin releasing test hydrogen peroxide oxidative damage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
