眼镜蛇中间克吕沃菌的分离鉴定及药敏试验 |
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引用本文: | 杜乐妍,王海烽,时晓,赵宝华,何宏轩.眼镜蛇中间克吕沃菌的分离鉴定及药敏试验[J].动物医学进展,2016(12). |
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作者姓名: | 杜乐妍 王海烽 时晓 赵宝华 何宏轩 |
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作者单位: | 1. 河北师范大学,河北石家庄 050024; 中国科学院动物研究所,北京 100101;2. 中国科学院动物研究所,北京,100101;3. 北京市水生野生动植物救护中心,北京,102100;4. 河北师范大学,河北石家庄,050024 |
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摘 要: | 为了研究北京市昌平区蛇场眼镜蛇的死亡原因,对眼镜蛇进行剖检、病理组织观察、病原菌的分离培养、生化鉴定、16SrRNA PCR以及致病力试验,确定致病菌为中间克吕沃菌。药敏试验结果显示,该菌对阿米卡星、庆大霉素、亚胺培南、美洛培南、头孢他啶、头孢噻肟、头孢吡肟、氨曲南、阿莫西林/克拉维酸、哌拉西林/他唑巴坦、左氧沙星高度敏感,对氨苄西林、氨苄西林/舒巴坦、复方新诺明、氯霉素、四环素、哌拉西林耐药。试验结果可为眼镜蛇感染中间克吕沃菌的治疗提供依据。
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关 键 词: | 眼镜蛇 中间克吕沃菌 分离鉴定 药敏试验 |
Isolation,Identification and Antimicrobial Susceptibility Test of Kluyvera intermedia in Cobra |
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Abstract: | This study focused on the bacterial pathogen that infected snakes.Kluyvera intermedia was isola-ted from sick cobra of snake farm in Changping district,Beijing,through postmortem,histopathological ex-amination,pathogen isolation,biochemical identification,16 S rRNA PCR,and pathogenicity test.The re-sult of antimicrobial susceptibility test showed that Kluyvera intermedia was highly sensitive to amikacin, gentamicin,gentamicin,meropenem,meropenem,cefotaxime,cefepime,cefepime,amoxicillin/clavulanate, piperacillin/tazobactam and levofloxacin;but resistant to ampicillin,ampicillin,chloramphenicol,trime-thoprim/sulfamethoxazole,tetracycline and piperacillin,which provided useful information for treatment of cobra infection with Kluyvera intermedia . |
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Keywords: | cobra Kluyvera intermedia isolation and identification antimicrobical susceptibility test |
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