| 巴西橡胶树磷脂酰肌醇转移蛋白cDNA的克隆及其序列分析 |
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| 引用本文： | 罗明武,邓柳红.巴西橡胶树磷脂酰肌醇转移蛋白cDNA的克隆及其序列分析[J].广西农业生物科学,2010(1):164-169. |
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| 作者姓名： | 罗明武 邓柳红 |
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| 作者单位： | 海南大学材料与化工学院;中国热带农业科学院热带生物技术研究所; |
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| 基金项目： | 华南热带农业大学科技基金(Rnd0638);;中国热带农业科学院科技基金(Rykj0420)共同资助 |
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| 摘 要： | 本文从巴西橡胶树（Hevea brasiliensis）差减cDNA文库中筛选到一个与磷脂酰肌醇转移蛋白（phos-phatidylinositol transfer protein）同源性较高的基因片段,并根据该基因片段序列信息,设计特异性引物,采用cDNA末端快速扩增技术RACE（rapid amplification of cDNA ends）进行差异片段的5＇和3＇端的扩增,并获得长度为1081bp的全长cDNA克隆R291（GenBank登陆号：AY589690）。序列分析表明,该基因包含702bp的开放阅读框,编码234个氨基酸,推测其蛋白质的分子量为26.8kD,等电点为6.51,有一个的跨膜螺旋区（氨基酸位点为83～103）。R291基因含有一个脂质结合保守区（Sec14p-like lipid-binding domain）,具有CRAL-TRIO脂质结合结构域,推测该基因是一个磷脂酰肌醇转移蛋白基因。该基因的克隆将为橡胶树磷脂酰肌醇代谢的研究奠定了基础,将有助于进一步了解磷脂酰肌醇代谢与胶乳再生之间的关系。
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| 关 键 词： | 巴西橡胶树 胶乳 磷脂酰肌醇转移蛋白 RACE 序列分析 |
Cloning and Sequence Analysis of Phosphatidylinositol Transfer Protein cDNA from Hevea brasilensis |
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| Luo Mingwu Deng Liuhong College of Materials Science , Chemical Engineering,Hainan University,Haikou.Cloning and Sequence Analysis of Phosphatidylinositol Transfer Protein cDNA from Hevea brasilensis[J].Journal of Guangxi Agricultural and Biological Science,2010(1):164-169. |
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| Authors: | Luo Mingwu Deng Liuhong College of Materials Science Chemical Engineering Hainan University Haikou |
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| Institution: | Luo Mingwu 1 Deng Liuhong2 1 College of Materials Science , Chemical Engineering,Hainan University,Haikou,570228,2 Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,571101 |
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| Abstract: | In this paper,a cDNA clone was isolated related to lipid transportation and metabolism by screening of a subtracted latex cDNA library of Hevea brasiliensis. According to its sequences information,a novel full-length cDNA termed R291 (GenBank number:AY589690) was obtained by using rapid amplification of cDNA ends (RACE). R291 was 1 081 bp long containing a 702 bp ORF,encoding 234 amino acids with a theoretical molecu lar weight of 26.8 kD and an isoelectric point of 6.51. In addition,hydropathy and transmem... |
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| Keywords: | Hevea brasiliensis Latex Phosphatidylinositol transfer protein RACE Sequence analysis |
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