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草莓果实采后NAD激酶活性与NAD(H)、NADP(H)含量及活性氧代谢的关系
引用本文:顾采琴,朱冬雪,李棋.草莓果实采后NAD激酶活性与NAD(H)、NADP(H)含量及活性氧代谢的关系[J].中国农业科学,2007,40(2):352-357.
作者姓名:顾采琴  朱冬雪  李棋
作者单位:1. 贵州大学食品科学系,贵阳,550025;广州大学食品科学系,广州,510405
2. 贵州大学农业生物工程重点实验室,贵阳,550025
3. 贵州大学食品科学系,贵阳,550025
基金项目:国家自然科学基金;贵州大学校科研和教改项目
摘    要:【目的】研究草莓采后成熟衰老过程中NADK活性与NAD(H)、NADP(H)及活性氧代谢和膜氧化产物变化的关系,以探讨NADK在非跃变型果实成熟衰老过程中的作用,为调控果实的成熟衰老提供理论依据。【方法】将从果园采回的草莓果实贮藏于不同的温度下并进行每天取样,研究草莓果实在低温(4℃)、常温(20℃)贮藏期间成熟衰老过程中NAD激酶(NADK)活性及其底物NAD(H)、产物NADP(H)以及超氧阴离子O2- ?过氧化氢(H2O2)、膜氧化产物丙二醛(MDA)含量的变化并分析NADK 与上述指标的关系。【结果】草莓果实在低温(4℃)贮藏时,其NADK活性比常温(20℃)贮藏的高,NAD(H)含量则相应比常温贮藏的低,NADP(H)含量则高于常温下的;同时,在常温贮藏期间果实O2- ?生成速率和H2O2含量、膜氧化产物MDA含量均比低温贮藏的高,暗示NADK可能通过影响NAD(H)、NADP(H)的含量及比例来调控O2- ?生成速率和H2O2含量,从而调控果实的成熟衰老。【结论】非跃变型果实草莓采后成熟衰老过程中,保持较高的NADK活性有利于延缓果实的成熟衰老,降低NADK活性可导致NAD和NAD(H)含量的积累,进而加速电子传递,产生大量的活性氧O2- ?和H2O2,从而促进膜过氧化作用和积累较多的MDA,最终导致果实衰老变质。

关 键 词:草莓  成熟衰老  NAD激酶  NAD(H)  NADP(H)  活性氧
收稿时间:2006-2-17
修稿时间:2006-03-10

Relationship Between NAD Kinase and NAD(H), NADP(H) and Active Oxygen during Ripening and Senescence of Postharvested Strawberry Fruit
GU Cai-qin,ZHU Dong-xue,LI Qi.Relationship Between NAD Kinase and NAD(H), NADP(H) and Active Oxygen during Ripening and Senescence of Postharvested Strawberry Fruit[J].Scientia Agricultura Sinica,2007,40(2):352-357.
Authors:GU Cai-qin  ZHU Dong-xue  LI Qi
Institution:1. Department of Food Science, Guizhou University, Guiyang 550025; 2. Department of Food Science, Guangzhou University, Guangzhou 510405; 3.Key Laboratory of Agriculture Biology Engineer, Guizhou University, Guiyang 550025
Abstract:Abstracts: The changes of NADK activity during ripening and senescence of posth- arvest strawberry fruit at 4℃ and 20℃ and the content of NAD(H) , NADP(H), H2O2 , malondialdehyde(MDA) and O2- production rate were studied . The results showed that the NADK activity and the contents of NADP(H) in strawberry at low temperature(4℃) was more than those at 20℃, but the content of NAD(H)at 4℃ was less than that at 20℃. Meanwhile, the O2- production rate and the content of H2O2 and MDA at 4℃ was slower and less than those at 20℃. It implied that NADK regulated the process of oxidation metabolism and ripening and senescence of strawberry probably by influencing the content and proportion of NAD(H)and NADP(H) , the O2- production rate and content of H2O2 .
Keywords:NAD(H)  NADP(H)
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