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河南省非洲猪瘟感染情况筛查
引用本文:杨霞,陈陆,孙彦婷,刘春生,常洪涛,郑鹿平,刘红英,王川庆.河南省非洲猪瘟感染情况筛查[J].中国农学通报,2010,26(21):1-5.
作者姓名:杨霞  陈陆  孙彦婷  刘春生  常洪涛  郑鹿平  刘红英  王川庆
作者单位:河南农业大学牧医工程学院,郑州,450002
基金项目:国家科技部支撑计划食品安全专项"畜禽及其制品安全生产的质量控制技术研究:重要人畜共患病快速检测技术研究与示范" 
摘    要:目的:对河南省猪瘟(CSF)流行区的猪群进行非洲猪瘟(ASF)感染的排查;方法:利用猪瘟抗原捕获ELISA试剂盒对27个猪场的猪瘟疑似病料进行CSFV鉴定,并用区别猪瘟强弱毒的RT-PCR检测方法鉴定猪瘟疑似病料。与此同时针对ASFV VP73蛋白基因设计一对引物利用PCR方法对ASFV进行排查;结果:河南省CSFV流行区没有ASFV的感染;且RT-PCR对CSFV的检出率高于ELISA ,两者差异显著。结果表明,尽管目前中国作为俄罗斯的邻国存在ASFV的威胁,但中国河南仍没有ASFV感染。

关 键 词:西洋参  西洋参  连作  酶活性  根系活力  
收稿时间:4/6/2010 12:00:00 AM
修稿时间:2010/5/21 0:00:00

Detection Of ASF Infection Condition In Henan
Yang Xia,Chen Lu,Sun Yanting,Liu Chunsheng,Chang Hongtao,Zheng Luping,Liu Hongying,Wang Chuanqing.Detection Of ASF Infection Condition In Henan[J].Chinese Agricultural Science Bulletin,2010,26(21):1-5.
Authors:Yang Xia  Chen Lu  Sun Yanting  Liu Chunsheng  Chang Hongtao  Zheng Luping  Liu Hongying  Wang Chuanqing
Institution:Yang Xia,Chen Lu,Sun Yanting,Liu Chunsheng,Chang Hongtao,Z heng Luping,Liu Hongying,Wang Chuanqing( Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002)
Abstract:Detection of ASF infection in CSF epidemiological area of Henan province; Suspected-CSFh istologic materials of 27 pig farms in Henan were detected and identified by the commercial antigen-captureE LISA assay and RT-PCR for detection and differentiation of wild-type and vaccine viruses of classical swinef ever virus (CSFV). At the same time, primers were designed according to the nucleotide sequence of VP73g ene of african swine fever virus(ASFV) and PCR assays were performed to screen ASF infection; None A...
Keywords:PCR
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