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| 半滑舌鳎外周血淋巴细胞培养条件研究 | |
| 引用本文： | 王美玉,刘海金,范兆廷,宋立民.半滑舌鳎外周血淋巴细胞培养条件研究[J].东北农业大学学报,2012,43(6):106-110. |
| 作者姓名： | 王美玉 刘海金 范兆廷 宋立民 |
| 作者单位： | 1. 东北农业大学动物科学技术学院,哈尔滨,150030 2. 中国水产学科学研究院,北京,100141 3. 中国水产学科学研究院北戴河中心试验站,河北秦皇岛,066100 |
| 基金项目： | 国家鲆鲽类产业技术体系项目 |
| 摘 要： | 以半滑舌鳎为试验对象,对淋巴细胞体外培养和染色体制备条件如促分裂剂、培养时间、培养温度、培养基、秋水仙素浓度及作用时间等进行探索,在建立一套基于半滑舌鳎淋巴细胞体外培养的染色体制备方法。结果表明,对于半滑舌鳎淋巴细胞体外培养,用离心法收集淋巴细胞,培养基为含15%体积分数胎牛血清的L-15培养基,添加3μg.mL-1的PHA-P作促分裂剂,分裂指数可达2.6%±0.2%,6 d后收集细胞制作染色体,获得较好效果。 |
| 关 键 词： | 半滑舌鳎 淋巴细胞培养 染色体制备 分裂指数 |
Study on blood lymphocytic cell cultivation of half smooth tongue sole ( Cynoglossus semilaevis) |
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| WANG Meiyu , LIU Haijin , FAN Zhaoting , SONG Limin.Study on blood lymphocytic cell cultivation of half smooth tongue sole ( Cynoglossus semilaevis)[J].Journal of Northeast Agricultural University,2012,43(6):106-110. | |
| Authors: | WANG Meiyu LIU Haijin FAN Zhaoting SONG Limin |
| Institution: | 1.School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;2.Chiese Academy of Fishery Sciences,Beijing 100141,China;3.Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao Hebei 066100,China) |
| Abstract: | Half smooth tongue sole(Cynoglossus semilaevis) was used to explore the condition of the peripheral blood lymphocytic cell cultivation and chromosome preparation.The conditions were searched from mitogens,incubation periods,culture temperature,media and the effect of the chloride.The aim was to establish a systemic method.The results showed that lymphocytes isolated from fresh blood by a stirring method were cultured in medium L-15 supplemented with 15%FBS,3 μg·mL-1 PHA(phytohemagglutinin) as mitogens,and collected at day 6.This was the optimized culture condition for half smooth tongue sole.After culture to make chromosome,the mitotic index(MI) could reach(2.6± 0.2)%. |
| Keywords: | Cynoglossus semilaevis lymphocyte culture chromosome preparation mitotic index |
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