| 园艺作物的ISSR分子标记研究及应用 |
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| 引用本文： | 刘淑芹,吴凤芝,刘守伟.园艺作物的ISSR分子标记研究及应用[J].东北农业大学学报,2012,43(4):145-150. |
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| 作者姓名： | 刘淑芹 吴凤芝 刘守伟 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | ISSR(Inter-simple sequence repeat)是一种基于微卫星序列发展起来的新型分子标记方法,具有无需知道任何靶标序列的微卫星背景信息、遗传多态性高、稳定高效、检测快速等特点。目前,ISSR分子标记技术在园艺作物的遗传多样性研究、品种鉴定、遗传图谱构建、基因定位及分子标记辅助育种等方面得到了广泛应用,文章就ISSR标记的原理、方法、特点及其在园艺作物研究中的应用加以综述。
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| 关 键 词： | ISSR 分子标记 园艺作物 应用 |
Inter-simple sequence repeat and its application in horticultural crop research |
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| LIU Shuqin
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WU Fengzhi
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LIU Shouwei.Inter-simple sequence repeat and its application in horticultural crop research[J].Journal of Northeast Agricultural University,2012,43(4):145-150. |
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| Authors: | LIU Shuqin WU Fengzhi LIU Shouwei |
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| Institution: | (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Inter-simple sequence repeat(ISSR) is a new molecular marker method which is based on micro-satellite technique with advantages of simple,quick,stable,reliable and higher DNA polymorphism,etc.At present,ISSR has been widely applied in genetic diversity research,genetic map construction,genetic mapping,molecular marker assisted breeding and variety purity identification of horticultural crops.The purpose of this review is to introduce principles,methods,characteristics of the ISSR and its application in the research of horticultural crops. |
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| Keywords: | inter-simple sequence repeat molecular marker horticultural crops application |
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