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蘑菇分离菌株(S1、S2)的ISSR分析
引用本文:关雪娟,许璐,邵彦春,王卓仁,陈福生,罗信昌.蘑菇分离菌株(S1、S2)的ISSR分析[J].中国食用菌,2007,26(3):41-43.
作者姓名:关雪娟  许璐  邵彦春  王卓仁  陈福生  罗信昌
作者单位:1. 湖北华中农业大学食品科技学院,武汉,430070
2. 湖北华中农业大学植物科技学院,武汉,430070
基金项目:中国食用菌协会资助项目
摘    要:利用简单重复序列间扩增多态性(ISSR)对12株蘑菇属菌株(其中菌株S1、S2为河北康保分离的菌株,具自主知识产权)和3株属外参照菌株进行了遗传多样性分析,构建了遗传相关聚类图。本研究结果表明,河北康保分离的两株菌株S1和S2与现存生产上应用的同类菌种存在明显差异,同时也表明ISSR分子标记在蘑菇品种的鉴别和菌株亲缘关系分析上具有较好的适用性。

关 键 词:蘑菇菌株  多态性  聚类分析
文章编号:1003-8310(2007)03-0041-03
修稿时间:2007-01-12

ISSR Analysis of Isolated Strains (S1, S2) of Agaricus Strains
GUAN Xue-Juan,XU Lu,SHAO Yan-Chun,WANG Zhuo-Ren,CHEN Fu-Sheng,LUO Xin-Chang.ISSR Analysis of Isolated Strains (S1, S2) of Agaricus Strains[J].Edible Fungi of China,2007,26(3):41-43.
Authors:GUAN Xue-Juan  XU Lu  SHAO Yan-Chun  WANG Zhuo-Ren  CHEN Fu-Sheng  LUO Xin-Chang
Institution:1. College of Food Science and Technology, Huazhang Agricultural Univrsity, Wuhan 430070; 2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070
Abstract:12 Agaricus strains (including 2 strains which were isolated from Kangbao in Hebei province with self-owned intellectual property rights) and 3 out-genus reference strains were analyzed for the genetic diversity using inter simple sequence repeats (ISSR). The results in this paper revealed that these two isolated strains S1 and S2 had great differences with those strains in the similar species which were used in production nowadays. ISSR was a suitable molecular marker for species differentiation and genetic analysis among close related strains of Agaricus strains.
Keywords:Agaricus strains  Polymorphism  Cluster analysis
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