| 双孢蘑菇湿泡病抗病性鉴定方法研究 |
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| 引用本文： | 张春兰,孙灿,金正文,曾云霞,徐玮琦,周晨,李玉.双孢蘑菇湿泡病抗病性鉴定方法研究[J].中国食用菌,2021(2):94-100,105. |
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| 作者姓名： | 张春兰 孙灿 金正文 曾云霞 徐玮琦 周晨 李玉 |
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| 作者单位： | 长春大学园林学院;吉林农业大学食药用菌教育部工程研究中心 |
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| 基金项目： | 吉林省教育厅科学技术研究规划项目(2021LY527L43)。 |
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| 摘 要： | 以3个不同颜色的双孢蘑菇品种,4株不同地理来源的有害疣孢霉菌株为供试材料,对有害疣孢霉的接种方式、接种浓度、接种时双孢蘑菇菌盖大小、接种后湿度控制及抗病性评价时间等因素做了综合研究及分析。观察并记录发病症状及发病率,建立病情评价标准。结果显示最佳评价方法为,喷雾式接种,菌盖直径9 mm~14 mm,接种有害疣孢霉的最佳孢子浓度3×104个/mL,接种后最佳培养湿度86%~91%,最佳评价时间为接种后第5天。建立科学的适用于双孢蘑菇湿泡病的抗病评价体系,为双孢蘑菇湿泡病抗病遗传育种工作的顺利开展提供科学前提。
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| 关 键 词： | 双孢蘑菇 有害疣孢霉菌 病症 抗病性 |
A Method to Evaluation the Resistance of Agaricus bisporus to Wet Bubble Disease |
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| ZHANG Chun-lan,SUN Can,JIN Zheng-wen,ZENG Yun-xia,XU Wei-qi,ZHOU Chen,LI Yu.A Method to Evaluation the Resistance of Agaricus bisporus to Wet Bubble Disease[J].Edible Fungi of China,2021(2):94-100,105. |
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| Authors: | ZHANG Chun-lan SUN Can JIN Zheng-wen ZENG Yun-xia XU Wei-qi ZHOU Chen LI Yu |
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| Institution: | (Changchun University,Changchun 130118,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agriculture University,Changchun 130118,China) |
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| Abstract: | In order to establish a stable resistance evaluation system of Agaricus bisporus wet bubble disease.Three cultures of A.bisporus and four isolates of Hypomyces perniciosus were selected as the materials.The inoculation concentrations,inoculation stages,and evaluation times were screened and disease symptoms recorded.The results were as follows,the optimal inoculation and disease resistance evaluation method was as follows:optimal concentration was 3×104 spore per milliltier inoculated on the surface of the peilus(peilus diameter is 9 mm-14 mm),after inoculation and cultivation under 86%-91%humidity,5 days later investigation of the disease index(DI)was done.The DI was determined by the number and size of visible blotches on the surface of the peilus. |
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| Keywords: | Agaricus bisporus Hypomyces perniciosus disease symptoms resistance |
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