High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China |
| |
| Authors: | Hong-Xia Jiang Dian-Hong Lü Zhang-Liu Chen Xiu-Mei Wang Ji-Rong Chen Ya-Hong Liu Xiao-Ping Liao Jian-Hua Liu Zhen-Ling Zeng |
| |
| Institution: | 1. Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, China;2. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China;3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450046, China;4. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;5. University of Chinese Academy of Sciences, Beijing 100049, China;6. Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-Food Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou, Jiangsu 225009, China;7. Beijing Key Laboratory of Antimicrobial Resistance and Pathogen Genomics, Beijing 100101, China;8. Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases First Attainted Hospital, College of Medicine, Zhejiang University, 310006, China;9. Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Sichuan 646000, China;1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Engineering Research of Veterinary Bio-products of Agricultural Ministry, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China;3. College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, China |
| |
| Abstract: | Escherichia coli play an important ecological role within resistant bacteria populations, and can be used as a bio-indicator of antimicrobial resistance. The aim of the present study was to use this feature of E. coli to investigate the prevalence of antimicrobial resistance and the degree of cross-species transmission of bacteria in pigs and poultry in China. A total of 592 E. coli strains, isolated from pigs and poultry (healthy and diseased animals), were tested for resistance to 22 antimicrobials representing eight antimicrobial drug types.E. coli isolates had high rates of resistance to ampicillin (99.5%), doxycycline (95.6%), tetracycline (93.4%), trimethoprim–sulfamethoxazole (74.3%), amoxicillin (65.1%), streptomycin (54.7%), and chloramphenicol (50.2%). Resistance to cephalosporins, quinolones, and aminoglycosides was also quite prevalent. The majority (81%) of isolates demonstrated multi-antimicrobial resistance, most commonly to 5–6 different antimicrobial types. One isolate was resistant to all 22 antimicrobials. Twenty-two cultures exhibiting multi-antimicrobial resistance were analysed by pulsed-field gel electrophoresis (PFGE) to assess their distribution between farms. Three distinct PFGE types were identified, indicating inter-farm transmission of multi-antimicrobial resistant bacteria. The study confirmed the presence and transmission of multi-antimicrobial-resistant E. coli strains amongst pigs and poultry in China and highlights the urgent need for appropriate monitoring programmes. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
