| 银耳子实体及菌丝体胶质化产生的双核酵母状孢子萌发条件探索 |
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| 引用本文： | 陈利丁,刘云超,孔旭强,张琪辉,纪君仪,李帆,孙淑静.银耳子实体及菌丝体胶质化产生的双核酵母状孢子萌发条件探索[J].中国食用菌,2021(2):11-18. |
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| 作者姓名： | 陈利丁 刘云超 孔旭强 张琪辉 纪君仪 李帆 孙淑静 |
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| 作者单位： | 福建农林大学生命科学学院;古田县食用菌研发中心 |
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| 基金项目： | 国家自然基金青年项目(31201669);福建省科技厅区域发展项目(2018N3018);2019-2020福建省现代化农业产业技术建设(闽农综(2019)144号);福建省教育厅中青年教师教育科研项目(JAT 170207);福建省种业工程(fjzycxny2017010)。 |
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| 摘 要： | 为探索由银耳(Tremella fuciformis)子实体、菌丝体胶质化产生的双核酵母状孢子(FBMds)的萌发条件,研究比较了不同来源和不同浓度的香灰菌(Annuloypoxylon stygium)浸提液及不同来源银耳孢子发酵液对银耳Tr21 FBMds萌发率的影响。试验结果表明,香灰菌浸提液和银耳孢子发酵液均有不同程度促银耳Tr21FBMds萌发的作用。不同浓度香灰菌浸提液对FBMds萌发率的试验表明,在一定浓度范围内,高浓度香灰菌浸提液可显著提高银耳FBMds萌发率,最佳浓度为40%;在该浓度下,银耳Tr21的原伴生香灰菌HTW01-5的浸提液对FBMds萌发率作用高达30.34%。银耳孢子T30-CAS、Tr01、Tr21、T8225-CAS的发酵液虽能显著促进银耳Tr21 FBMds的萌发,但效果不如香灰菌浸提液明显。研究所得银耳菌丝为纯菌丝,可稳定传代,为进一步研究银耳二型态转变以及银耳新品种选育奠定了扎实基础。
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| 关 键 词： | 银耳孢子 香灰菌浸提液 孢子发酵液 萌发 |
Conditions for Germination of Dikaryotic Yeast-like Spores from Fruiting Body and Mycelium of Tremella fuciformis |
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| CHEN Li-ding,LIU Yun-chao,KONG Xu-qiang,ZHANG Qi-hui,JI Jun-yi,LI Fan,SUN Shu-jing.Conditions for Germination of Dikaryotic Yeast-like Spores from Fruiting Body and Mycelium of Tremella fuciformis[J].Edible Fungi of China,2021(2):11-18. |
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| Authors: | CHEN Li-ding LIU Yun-chao KONG Xu-qiang ZHANG Qi-hui JI Jun-yi LI Fan SUN Shu-jing |
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| Institution: | (College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Gutian Edible Fungi Research and Development Center,Ningde 352200,China) |
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| Abstract: | In order to explore the germination conditions of Tremella fuciformis FBMds(dikaryotic yeast-like spores from fruiting body and mycelia),we compared the effects of different sources and concentration of the extract of Annuloypoxylon stygium on the germination rate of Tr21 FBMds,and also compared the effects of different sources of the fermentation broth of T.fuciformis spores on the germination rate of Tr21 FBMds.The results showed that the extract of A.stygium and the fermentation broth of T.fuciformis spores could promote the germination of Tr21 FBMds.The experimental results of different concentration A.stygium extract on germination rate of FBMds indicate that the germination rate of FBMds could be significantly increased by the high concentration of A.stygium,and the best gemination-promoting concentration was 40%.At this concentration,the effect of HTW01-5 extract on the germination rate of FBMds was as high as 30.34%,and the strain of HTW01-5 is original companion fungus of T.fuciformis Tr21.The promoting effects were also showed when adding the fermentation broths of T.fuciformis spores T30-CAS,Tr01,F21,T8225-CAS,but less than adding the A.stygium extract.The T.fuciformis mycelium obtained in this study is pure and passed on stably,which lays a solid foundation for further study on the transformation of dimorphism of T.fuciformis and the breeding of new varieties of T.fuciformis. |
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| Keywords: | Tremella fuciformis spores extracts of Annuloypoxylon stygium fermentation broth of spores germination |
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