Collection of Lupinus angustifolius L. Germplasm and Characterisation of Morphological and Molecular Diversity |
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| Authors: | Pedro Talhinhas José Leitão João Neves-Martins |
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| Institution: | (1) DBEB, Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal;(2) FERN, Universidade do Algarve, 8000-117 Faro, Portugal |
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| Abstract: | Lupinus angustifolius L. is a Mediterranean species, domesticated in the 20th century, representing an important grain legume crop in Australia
and other countries. This work is focused on the collection of wild germplasm and on the characterisation of morphological
and molecular diversity of germplasm accessions. It reports the collection of 81 wild L. angustifolius accessions from the South and Centre of Portugal, available at the ‘Instituto Superior de Agronomia Gene Bank’, with subsequent
morphological and molecular characterisation of a selection of these and other accessions. A multivariate analysis of morphological
traits on 88 L. angustifolius accessions (including 59 wild Portuguese accessions, 15 cultivars and 14 breeding lines) showed a cline of variation on wild
germplasm, with plants from Southern Portugal characterised by earlier flowering, higher vegetative development and larger
seeds. AFLP and ISSR molecular markers grouped modern cultivars as sub-clusters within the wider diversity of wild germplasm,
revealing the narrow pool of genetic diversity on which domesticated accessions are based. The importance of preserving, characterising
and using wild genetic resources for L. angustifolius crop improvement is outlined by the results obtained. |
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| Keywords: | AFLP Genetic diversity Germplasm collection ISSR Lupinus angustifolius Portugal |
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