| 猪乙型脑炎病毒RT-PCR检测方法的建立 |
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| 引用本文： | 车勇良,陈少莺,魏宏,王隆柏,陈仕龙,周伦江,庄向生.猪乙型脑炎病毒RT-PCR检测方法的建立[J].福建农业学报,2006,21(3):228-230. |
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| 作者姓名： | 车勇良 陈少莺 魏宏 王隆柏 陈仕龙 周伦江 庄向生 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建,福州,350013;福建省畜禽疫病防治工程技术研究中心,福建,福州,350013
2. 福建省农业科学院畜牧兽医研究所,福建,福州,350013 |
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| 摘 要： | 根据猪乙型脑炎病毒(JEV)基因序列,设计合成了一对引物,以JEV疫苗株为模板,建立了检测JEV的RT PCR方法。应用该方法对JEV疫苗株RNA进行扩增,获得与预期大小相符,长度为430 bp的特异性目的片段;RT PCR产物测序结果与文献报道的JEV不同毒株的序列同源性达到98%~100%;敏感性测定该RT PCR可扩增到10 pg的JEV-RNA。结果表明,建立的RT PCR方法对JEV的检测敏感性高、特异性强。
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| 关 键 词： | 乙型脑炎病毒 RT-PCR 检测 |
| 文章编号： | 1008-0384(2006)03-0228-03 |
| 修稿时间： | 2006年5月29日 |
Development of RT-PCR method for detecting swine janpanese encephalitis virus |
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| CHE Yong-liang,CHEN Shao-ying,WEI Hong,WANG Long-bai,CHEN Shi-long,ZHOU Lun-jiang,ZHUANG Xiang-sheng.Development of RT-PCR method for detecting swine janpanese encephalitis virus[J].Fujian Journal of Agricultural Sciences,2006,21(3):228-230. |
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| Authors: | CHE Yong-liang CHEN Shao-ying WEI Hong WANG Long-bai CHEN Shi-long ZHOU Lun-jiang ZHUANG Xiang-sheng |
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| Abstract: | A pair of primers were designed and synthesized based on the sequences of the JEV genome,and the RT-PCR method to detect the Japanese Encephalitis Virus(JEV) were development.The 430 bp specifical fragment were obtained from the JEV vaccine strains RNA by this RT-PCR.The test of the gene sequence of RT-PCR product achieved to 98%-100% with that of other JEV strains.The sensitivity of RT-PCR reached to 10 pg JEV RNA.These results showed that the RT-PCR method to detect JEV were of better specificity and higher sensibility. |
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| Keywords: | japanese encephalitis virus RT-PCR detection |
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