| 高大山羊草1Sl染色体特异分子标记的开发与应用 |
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| 引用本文： | 刘晓明,张姝倩,宫文英,唐海田,王灿国,程敦公,刘成,刘建军.高大山羊草1Sl染色体特异分子标记的开发与应用[J].农业科学与技术,2016(3):490-493. |
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| 作者姓名： | 刘晓明 张姝倩 宫文英 唐海田 王灿国 程敦公 刘成 刘建军 |
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| 作者单位： | 1. 潍坊科技学院生物工程研发中心,山东潍坊,262700;2. 潍坊科技学院化工与环境学院,山东潍坊,262700;3. 山东省农业科学院作物研究所,山东济南,250100;4. 烟台海洋环境监测中心站,山东烟台,264006 |
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| 基金项目： | National Natural Science Foundation of China(31201203)，Earmarked Fund for Modern Agro-industry Technology Research System(CARS-03-1-8)，China Postdoctoral Science Foundation(2013T60850)，Program for Youth Talent of Shandong Academy of Agricultural Sciences(1-18-024)，Seed Industry Foundation Grant to Taishan Scholar and Agricultural Improved Variety Industrialization Project of Shandong Province (2-B-08).基金项目国家自然科学基金项目(31201203)；国家现代农业产业技术体系建设专项(CARS-03-1-8)；中国博士后基金项目(2013T60850)；山东省农业科学院青年拔尖人才科研启动项目( |
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| 摘 要： | 导入小麦的高大山羊草（Aegilops longissima）1Sl染色体可以显著提高小麦加工品质及子粒Fe和 Zn元素含量。因此，1Sl染色体特异分子标记的建立对子粒 Fe和 Zn元素含量高的高品质小麦的选育具有重要意义。试验建立了高大山羊草1Sl染色体特异分子标记9个。其中染色体短臂标记2个，长臂标记6个，同时定位于长臂和短臂的标记1个。利用建立的分子标记对杂交群体进行检测。结果表明，建立的9个标记可以对分离群体进行有效筛选与鉴定。因此，获得的高大山羊草1Sl染色体特异分子标记可以应用于杂交群体的筛选、鉴定以及辅助选育子粒 Fe和 Zn元素含量高的高品质小麦。
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| 关 键 词： | 高大山羊草(Aegilops longissima) 1Sl染色体 分子标记 小麦育种 |
Development and Utilization of 1Sl Chromosome-arm-specific Molecular Markers of Aegilops longissima |
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| Abstract: | Introducing the 1Sl chromosome of Aegilops longissima into wheat genome can significantly improve wheat grain quality and contents of iron and zinc. There-fore, the development of molecular markers specific to 1Sl chromosome of A. longis-sima is of important significance for breeding high-quality wheat with high contents of iron and zinc in grains. In this study, nine molecular markers specific to 1Sl chromosome of A. longissima were developed, including two 1SlS specific markers, six 1SlL specific markers and one 1Sl specific marker which was located on both short and long arms. The practicability of these molecular markers were verified us-ing hybrid population as materials. The results showed that hybrid population could be effectively screened and identified, which indicated that the developed 1Sl chro-mosome-specific molecular markers could be used for screening and identification of hybrid population and could be used in marker-assisted breeding of high-quality wheat with high contents of Fe and Zn in grains. |
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| Keywords: | Aegilops longissima 1Sl chromosome Molecular marker Wheat breed-ing |
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