| 人工诱导白木香树体抗逆能力的研究 |
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| 引用本文： | 王东光,张宁南,杨曾奖,刘小金,洪舟,徐大平.人工诱导白木香树体抗逆能力的研究[J].华南农业大学学报,2016,37(6):70-76. |
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| 作者姓名： | 王东光 张宁南 杨曾奖 刘小金 洪舟 徐大平 |
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| 作者单位： | 中国林业科学研究院热带林业研究所,广东广州,510520 |
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| 基金项目： | 国家林业局林业公益性行业科研专项(201304402-4)；广东省林业科技创新项目(2014KJCX004-01) |
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| 摘 要： | 【目的】探索激素、盐、真菌和物理诱导对白木香Aquilaria sinensis树体抗逆能力的影响,揭示白木香树体结香机制。【方法】于广东省惠东县,采用随机区组设计,每处理设置3株单株重复。取促进期为6个月的木材样品,进行抗逆相关物质和酶活性的测定分析。【结果】激素、盐、真菌和物理类处理中,处理2(MeJA和Et),处理8NaHSO_3(w=1‰)、NaCl(w=20‰)和FeCl_2(w=10‰)],处理10黑绿木霉Trichoderma atroviride]和处理16(火烧孔)的白木香树体挥发油质量分数分别为8.48%、9.86%、12.46%和6.21%,接近或已达沉香入药标准(10%)。其总酚、可溶性糖质量分数和CAT、POD、SOD、NOS、PAL活性均高于同类处理,MDA和淀粉质量分数均低于同类处理。主成分分析表明,处理2、8、10和16白木香树体抗逆能力综合得分为1.83、2.31、2.10和-1.51,分别为同类处理最高。【结论】Me JA和Et,NaHSO_3(w=1‰)、NaCl(w=20‰)和FeCl_2(w=10‰),黑绿木霉和火烧孔处理白木香树体抗逆能力强,有益于树体结香。
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| 关 键 词： | 白木香 人工诱导 抗逆 结香 |
| 收稿时间： | 2016/1/17 0:00:00 |
Study on resistance ability of Aquilaria sinensis trees under artificial induction |
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| WANG Dongguang,ZHANG Ningnan,YANG Zengjiang,LIU Xiaojin,HONG Zhou and XU Daping.Study on resistance ability of Aquilaria sinensis trees under artificial induction[J].Journal of South China Agricultural University,2016,37(6):70-76. |
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| Authors: | WANG Dongguang ZHANG Ningnan YANG Zengjiang LIU Xiaojin HONG Zhou and XU Daping |
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| Institution: | Research Institute of Tropical Forestry, Chinese Academy of Forestry,,Research Institute of Tropical Forestry, Chinese Academy of Forestry,,Research Institute of Tropical Forestry, Chinese Academy of Forestry,,Research Institute of Tropical Forestry, Chinese Academy of Forestry,,Research Institute of Tropical Forestry, Chinese Academy of Forestry, and Research Institute of Tropical Forestry, Chinese Academy of Forestry, |
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| Abstract: | Objective] To study the effects of hormone, salt, fungi and artificial wounding induction on resistance ability of Aquilaria sinensis trees, and discover the mechanism of agarwood formation in A. sinensis trees.Method]We conducted the experiment in Huidong county, Guangdong province, and used a random block design with three single tree repeats per treatment.Six months after stimulation, stress-resistance related substances and enzyme activities were analyzed for A.sinensis tree samples.Result]Among the different treatments of hormone, salt, fungi and artificial wounding, the optimal treatments were treatment 2 (MeJA and Et), treatment 8 NaHSO3(w=1‰), NaCl(w=20‰) and FeCl2(w=10‰) ] , treatment 10 ( Trichoderma atroviride ) and treatment 16 ( burning-chisel-drilling ) .For these four treatments, the contents of essential oil were 8.48%, 9.86%, 12.46% and 6.21% respectively, approaching or reaching agarwood quality standard ( 10%) .Polyphenol, soluble sugar content, and CAT, POD, SOD, NOS, PAL activities were higher compared to other treatments of the same treatment type, while MDA and starch contents were relatively lower.Based on principal component analysis, the scores of resistance ability of A.sinensis trees with treatment 2, 8, 10 and 16 were 1.83,2.31,2.10 and-1.51, respectively, all highest among the same treatment type.Conclusion] MeJA and Et, NaHSO3 ( w=1‰) , NaCl ( w=20‰) and FeCl2 ( w=10‰) , Trichoderma atroviride and burning-chisel-drilling could induce strong resistance, and could promote agarwood formation of A.sinensis trees. |
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| Keywords: | Aquilaria sinensis artificial induction stress resistance agarwood formation |
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